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Aging Reduces Insulin Clearance in Mice
- Source :
- Frontiers in Endocrinology, Vol 12 (2021), Frontiers in Endocrinology
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- Hyperinsulinemia is frequently associated with aging and may cause insulin resistance in elderly. Since insulin secretion and clearance decline with age, hyperinsulinemia seems to be maintained, primarily, due to a decrease in the insulin clearance. To investigate these aging effects, 3- and 18-month-old male C57BL/6 mice were subjected to intraperitoneal glucose and insulin tolerance tests (ipGTT and ipITT) and, during the ipGTT, plasma c-peptide and insulin were measure to evaluate in vivo insulin clearance. Glucose-stimulated insulin secretion in isolated pancreatic islets was also assessed, and liver samples were collected for molecular analyses (western blot). Although insulin sensitivity was not altered in the old mice, glucose tolerance, paradoxically, seems to be increased, accompanied by higher plasma insulin, during ipGTT. While insulin secretion did not increase, insulin clearance was reduced in the old mice, as suggested by the lower c-peptide:insulin ratio, observed during ipGTT. Carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) and insulin-degrading enzyme (IDE), as well as the activity of this enzyme, were reduced in the liver of old mice, justifying the decreased insulin clearance observed in these mice. Therefore, loss of hepatic CEACAM1 and IDE function may be directly related to the decline in insulin clearance during aging.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Aging
insulin secretion
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Insulysin
Diseases of the endocrine glands. Clinical endocrinology
03 medical and health sciences
Islets of Langerhans
Mice
0302 clinical medicine
Insulin resistance
Endocrinology
Western blot
In vivo
Antigens, CD
Internal medicine
Insulin-degrading enzyme
Hyperinsulinemia
Medicine
Animals
Insulin
insulin sensitivity
CEACAM1
Original Research
chemistry.chemical_classification
medicine.diagnostic_test
business.industry
Pancreatic islets
Glucose Tolerance Test
medicine.disease
hepatic insulin clearance
RC648-665
insulin-degrading enzyme
030104 developmental biology
Enzyme
medicine.anatomical_structure
Glucose
chemistry
Liver
hyperinsulinemia
Insulin Resistance
business
Cell Adhesion Molecules
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 16642392
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Endocrinology
- Accession number :
- edsair.doi.dedup.....994dd1d23eef448df8c3614b91cd2c4d
- Full Text :
- https://doi.org/10.3389/fendo.2021.679492/full