Back to Search Start Over

Pentaketide Resorcylic Acid Synthesis by Type III Polyketide Synthase from Neurospora crassa

Pentaketide Resorcylic Acid Synthesis by Type III Polyketide Synthase from Neurospora crassa

Authors :
Takayoshi Awakawa
Sueharu Horinouchi
Nobutaka Funa
Source :
Journal of Biological Chemistry. 282:14476-14481
Publication Year :
2007
Publisher :
Elsevier BV, 2007.

Abstract

Type III polyketide synthases (PKSs) are responsible for aromatic polyketide synthesis in plants and bacteria. Genome analysis of filamentous fungi has predicted the presence of fungal type III PKSs, although none have thus far been functionally characterized. In the genome of Neurospora crassa, a single open reading frame, NCU04801.1, annotated as a type III PKS was found. In this report, we demonstrate that NCU04801.1 is a novel type III PKS catalyzing the synthesis of pentaketide alkylresorcylic acids. NCU04801.1, hence named 2'-oxoalkylresorcylic acid synthase (ORAS), preferred stearoyl-CoA as a starter substrate and condensed four molecules of malonyl-CoA to give a pentaketide intermediate. For ORAS to yield pentaketide alkylresorcylic acids, aldol condensation and aromatization of the intermediate, which is still attached to the enzyme, are presumably followed by hydrolysis for release of the product as a resorcylic acid. ORAS is the first type III PKS that synthesizes pentaketide resorcylic acids.

Details

ISSN :
00219258
Volume :
282
Database :
OpenAIRE
Journal :
Journal of Biological Chemistry
Accession number :
edsair.doi.dedup.....99481dea16f4526a42758ba46350cbcc
Full Text :
https://doi.org/10.1074/jbc.m701239200