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Pentaketide Resorcylic Acid Synthesis by Type III Polyketide Synthase from Neurospora crassa
Pentaketide Resorcylic Acid Synthesis by Type III Polyketide Synthase from Neurospora crassa
- Source :
- Journal of Biological Chemistry. 282:14476-14481
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Type III polyketide synthases (PKSs) are responsible for aromatic polyketide synthesis in plants and bacteria. Genome analysis of filamentous fungi has predicted the presence of fungal type III PKSs, although none have thus far been functionally characterized. In the genome of Neurospora crassa, a single open reading frame, NCU04801.1, annotated as a type III PKS was found. In this report, we demonstrate that NCU04801.1 is a novel type III PKS catalyzing the synthesis of pentaketide alkylresorcylic acids. NCU04801.1, hence named 2'-oxoalkylresorcylic acid synthase (ORAS), preferred stearoyl-CoA as a starter substrate and condensed four molecules of malonyl-CoA to give a pentaketide intermediate. For ORAS to yield pentaketide alkylresorcylic acids, aldol condensation and aromatization of the intermediate, which is still attached to the enzyme, are presumably followed by hydrolysis for release of the product as a resorcylic acid. ORAS is the first type III PKS that synthesizes pentaketide resorcylic acids.
- Subjects :
- Biology
Biochemistry
Mass Spectrometry
Neurospora crassa
Lactones
Hydrolysis
Polyketide
Cloning, Molecular
Molecular Biology
chemistry.chemical_classification
Molecular Structure
ATP synthase
Resorcinols
Cell Biology
biology.organism_classification
Salicylates
Kinetics
Open reading frame
Enzyme
chemistry
biology.protein
Aldol condensation
Acyl Coenzyme A
Chromatography, Thin Layer
Polyketide Synthases
Bacteria
Chromatography, Liquid
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 282
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....99481dea16f4526a42758ba46350cbcc
- Full Text :
- https://doi.org/10.1074/jbc.m701239200