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Electrochemiluminescence Biosensor Based on 3-D DNA Nanomachine Signal Probe Powered by Protein-Aptamer Binding Complex for Ultrasensitive Mucin 1 Detection
- Source :
- Analytical chemistry. 89(7)
- Publication Year :
- 2017
-
Abstract
- Herein, we fabricated a novel electrochemiluminescence (ECL) biosensor for ultrasensitive detection of mucin 1 (MUC1) based on a three-dimensional (3-D) DNA nanomachine signal probe powered by protein-aptamer binding complex. The assembly of 3-D DNA nanomachine signal probe achieved the cyclic reuse of target protein based on the protein-aptamer binding complex induced catalyzed hairpin assembly (CHA), which overcame the shortcoming of protein conversion with enzyme cleavage or polymerization in the traditional examination of protein. In addition, CoFe2O4, a mimic peroxidase, was used as the nanocarrier of the 3-D DNA nanomachine signal probe to catalyze the decomposition of coreactant H2O2 to generate numerous reactive hydroxyl radical OH• as the efficient accelerator of N-(aminobutyl)-N-(ethylisoluminol) (ABEI) ECL reaction to amplify the luminescence signal. Simultaneously, the assembly of 3-D DNA nanomachine signal probe was executed in solution, which led to abundant luminophore ABEI be immobilized a...
- Subjects :
- Luminescence
Surface Properties
Aptamer
Nanotechnology
02 engineering and technology
Biosensing Techniques
010402 general chemistry
01 natural sciences
Signal
Ferric Compounds
Catalysis
Analytical Chemistry
chemistry.chemical_compound
Electrochemiluminescence
Humans
Binding site
Particle Size
Binding Sites
Chemistry
Mucin-1
Cobalt
Electrochemical Techniques
Aptamers, Nucleotide
021001 nanoscience & nanotechnology
Combinatorial chemistry
0104 chemical sciences
Luminophore
Nanoparticles
Target protein
0210 nano-technology
DNA Probes
Biosensor
DNA
Subjects
Details
- ISSN :
- 15206882
- Volume :
- 89
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....9922dc035600943afec6877ac3dc142d