Back to Search
Start Over
Effects of miR-1236-3p and miR-370-5p on activation of p21 in various tumors and its inhibition on the growth of lung cancer cells
- Source :
- Tumor Biology, Vol 39 (2017)
- Publication Year :
- 2017
-
Abstract
- The mechanism of dsRNA-induced gene activation (RNAa) is being gradually unveiled. The plentiful evidence that it existed in mammalian species other than human demonstrated that dsRNA-mediated RNAa is a conservative phenomenon. Simultaneously, accumulating evidence suggested that microRNAs could activate gene expression by targeting promoter. Nevertheless, it is ambiguous whether microRNA-induced gene activation in different human cells is a common phenomenon. The study we performed verified that miR-1236-3p (miR-1236) and miR-370-5p can activate p21 expression in bladder cancer (BCa) T24, EJ cells, and non-small-cell lung carcinoma A549 cells, while in hepatocellular HepG2 cells both microRNAs cannot effectively induce the expression of P21 WAF1/CIP1 (p21). In pancreatic cancer PANC-1 cells, only miR-370-5p had the potent abilities to induce p21 expression rather than miR-1236-3p. Unlike microRNA-mediated RNA activation, we can observe that dsP21-322 significantly activated p21 in above cells. Besides, we demonstrated that miR-1236 and miR-370 inhibited cyclin D1-CDK4/CDK6 pathway while upregulated E-cadherin expression by upregulation of p21 . Overexpression of these two microRNAs in A549 induced cell-cycle arrest and cell senescence, delayed cell proliferation and colony formation, and inhibited migration and invasion. In conclusion, microRNA-mediated RNAa depends on the cell context, and miR-1236 and miR-370 can inhibit non-small-cell lung carcinoma cell growth by upregulating p21 expression in vitro.
- Subjects :
- 0301 basic medicine
Cyclin-Dependent Kinase Inhibitor p21
Lung Neoplasms
Cell
RNA activation
03 medical and health sciences
Cancer stem cell
microRNA
medicine
Humans
Promoter Regions, Genetic
RC254-282
Cell Proliferation
Regulation of gene expression
A549 cell
biology
Cell growth
Chemistry
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
General Medicine
Cell Cycle Checkpoints
Hep G2 Cells
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Urinary Bladder Neoplasms
A549 Cells
biology.protein
Cancer research
Cyclin-dependent kinase 6
Subjects
Details
- ISSN :
- 14230380
- Volume :
- 39
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....9921b2413f103305c155b45f5a2d9fc9