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Differential effects of tamoxifen and I? on three distinguishable chloride currents activated in T84 intestinal cells
- Source :
- Pfl�gers Archiv European Journal of Physiology. 425:552-554
- Publication Year :
- 1993
- Publisher :
- Springer Science and Business Media LLC, 1993.
-
Abstract
- The whole-cell mode of the patch-clamp technique has been used to monitor ionic currents in T84 colonic carcinoma cells. The cells were stimulated by either a cAMP cocktail, ionomycin or hypotonicity. Sizeable currents with distinct kinetics were observed after the stimulation with the different agonists. These kinetically distinct Cl- currents also presented a differential sensitivity to the anti-oestrogen Tamoxifen and to the halide I-. Tamoxifen only inhibits the volume activated Cl- current without affecting the other two. Substitution of extracellular Cl- by I- shifted the reversal potential towards more negative values both in the hypotonicity and ionomycin activated Cl- currents. The cAMP activated current responded to the Cl- substitution by I- with a blockade of both outward and inward currents, in addition to the displacement of the zero current level towards positive values. Thus, the use of these two simple tools, I- and tamoxifen, allows the distinction of Cl- channels in epithelial cells.
- Subjects :
- medicine.medical_specialty
Physiology
Clinical Biochemistry
Kinetics
Iodide
Stimulation
chemistry.chemical_compound
Chlorides
Physiology (medical)
Internal medicine
Cyclic AMP
Tumor Cells, Cultured
Extracellular
medicine
Receptor
Reversal potential
chemistry.chemical_classification
Ionomycin
Carcinoma
Electric Conductivity
Iodides
Membrane transport
Tamoxifen
Endocrinology
Hypotonic Solutions
chemistry
Colonic Neoplasms
Biophysics
Subjects
Details
- ISSN :
- 14322013 and 00316768
- Volume :
- 425
- Database :
- OpenAIRE
- Journal :
- Pfl�gers Archiv European Journal of Physiology
- Accession number :
- edsair.doi.dedup.....991d2ffa94a13e5bd5c7fb86ee3bdf3f