Back to Search
Start Over
Broad-spectrum monoclonal antibodies against chikungunya virus structural proteins: Promising candidates for antibody-based rapid diagnostic test development
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 12, p e0208851 (2018)
- Publication Year :
- 2018
-
Abstract
- In response to the aggressive global spread of the mosquito-borne chikungunya virus (CHIKV), an accurate and accessible diagnostic tool is of high importance. CHIKV, an arthritogenic alphavirus, comprises three genotypes: East/Central/South African (ECSA), West African (WA), and Asian. A previous rapid immunochromatographic (IC) test detecting CHIKV E1 protein showed promising performance for detection of the ECSA genotype. Unfortunately, this kit exhibited lower capacity for detection of the Asian genotype, currently in circulation in the Americas, reflecting the low avidity of one of the monoclonal antibodies (mAbs) in this IC kit for the E1 protein of the Asian-genotype because of a variant amino acid sequence. To address this shortcoming, we set out to generate a new panel of broad- spectrum mouse anti-CHIKV mAbs using hybridoma technology. We report here the successful generation of mouse anti-CHIKV mAbs targeting CHIKV E1 and capsid proteins. These mAbs possessed broad reactivity to all three CHIKV genotypes, while most of the mAbs lacked cross-reactivity towards Sindbis, dengue, and Zika viruses. Two of the mAbs also lacked cross-reactivity towards other alphaviruses, including O'nyong-nyong, Ross River, Mayaro, Western Equine Encephalitis, Eastern Equine Encephalitis, and Venezuelan Equine Encephalitis viruses. In addition, another two mAbs cross-reacted weakly only with most closely related O'nyong-nyong virus. Effective diagnosis is one of the keys to disease control but to date, no antibody-based rapid IC platform for CHIKV is commercially available. Thus, the application of the mAbs characterized here in the rapid diagnostic IC kit for CHIKV detection is expected to be of great value for clinical diagnosis and surveillance purposes.
- Subjects :
- 0301 basic medicine
RNA viruses
Viral Diseases
Physiology
Cell Lines
medicine.disease_cause
Pathology and Laboratory Medicine
Biochemistry
Dengue fever
Viral Packaging
Mice
Immune Physiology
Medicine and Health Sciences
Chikungunya
Cross Reactivity
Multidisciplinary
Chikungunya Virus
Immune System Proteins
biology
virus diseases
Antibodies, Monoclonal
Infectious Diseases
Capsid
Medical Microbiology
Viral Pathogens
Viral Envelope
Viruses
Medicine
Biological Cultures
Antibody
Pathogens
Engineering sciences. Technology
Research Article
Neglected Tropical Diseases
medicine.drug_class
Science
Alphaviruses
030106 microbiology
Immunology
Alphavirus
Viral Structure
Monoclonal antibody
Research and Analysis Methods
Microbiology
Virus
Antibodies
Togaviruses
03 medical and health sciences
Viral Proteins
Virology
medicine
Animals
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Vero Cells
Biology and life sciences
Diagnostic Tests, Routine
Organisms
Chikungunya Infection
Proteins
medicine.disease
biology.organism_classification
Tropical Diseases
Viral Replication
030104 developmental biology
biology.protein
Hybridoma technology
Chikungunya Fever
Cloning
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....990361cf588b2ae2af2c7d800ffee661