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Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosynthesis
- Source :
- Molecular Plant. 8:911-921
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- The membrane lipids from fast-elongating wild-type cotton ( Gossypium hirsutum ) fibers at 10 days post-anthesis, wild-type ovules with fiber cells removed, and ovules from the fuzzless - lintless mutant harvested at the same age, were extracted, separated, and quantified. Fiber cells contained significantly higher amounts of phosphatidylinositol (PI) than both ovule samples with PI 34:3 being the most predominant species. The genes encoding fatty acid desaturases ( Δ 15 GhFAD ), PI synthase ( PIS ) and PI kinase ( PIK ) were expressed in a fiber-preferential manner. Further analysis of phosphatidylinositol monophosphate (PIP) indicated that elongating fibers contained four- to five-fold higher amounts of PIP 34:3 than the ovules. Exogenously applied linolenic acid (C18:3), soybean L-α-PI, and PIPs containing PIP 34:3 promoted significant fiber growth, whereas a liver PI lacking the C18:3 moiety, linoleic acid, and PIP 36:2 were completely ineffective. The growth inhibitory effects of carbenoxolone, 5-hydroxytryptamine, and wortmannin were reverted by C18:3, PI, or PIP, respectively, suggesting that PIP signaling is essential for fiber cell growth. Furthermore, cotton plants expressing virus-induced gene-silencing constructs that specifically suppressed GhΔ 15 FAD , GhPIS , or GhPIK expression, resulted in significantly short-fibered phenotypes. Our data provide the basis for in-depth studies on the roles of PI and PIP in mediating cotton fiber growth.
- Subjects :
- Dietary Fiber
Gossypium
Linolenic acid
Kinase
Membrane lipids
Linoleic acid
alpha-Linolenic Acid
Plant Science
Biology
Phosphatidylinositols
Biosynthetic Pathways
Wortmannin
chemistry.chemical_compound
Phosphatidylinositol Phosphates
Fiber cell
chemistry
Biosynthesis
Biochemistry
Gene Expression Regulation, Plant
lipids (amino acids, peptides, and proteins)
Phosphatidylinositol
Molecular Biology
Plant Proteins
Subjects
Details
- ISSN :
- 16742052
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecular Plant
- Accession number :
- edsair.doi.dedup.....98e50c781c265f4d6ed963f470e7aba0