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Glucagon-like peptide-2 reduces the obesity-associated inflammation in the brain
- Source :
- Neurobiology of Disease, Vol 121, Iss, Pp 296-304 (2019)
- Publication Year :
- 2018
-
Abstract
- Growing evidence suggests a link between obesity and neurodegeneration. The purpose of the present study was to explore the neuroprotective potential of glucagon-like peptide-2 (GLP-2) in the brain of high fat diet (HFD)-fed mice. Markers of inflammation and oxidative stress were analysed in the brains of obese mice chronically treated with [Gly2]-GLP-2 (teduglutide), the stable analogue of the GLP-2, and they were compared to age-matched untreated obese and lean animals. Neurodegeneration was examined by TUNEL assay. HFD feeding increased the expression of pro-inflammatory mediators (NF-kB, IL-8, TNF-α, IL-1β and IL-6), glial fibrillary acidic protein (GFAP), index of gliosis and neurodegeneration, stress marker proteins (p-ERK, Hsp60 and i-NOS), amyloid-β precursor protein (APP). [Gly2]-GLP-2 treatment significantly attenuated the HFD-induced increased expression of the various markers, as well as the higher levels of reactive oxygen species found in brains of untreated-HFD mice. Immunofluorescence confirmed that the increase of GFAP or APP in the brain cortex of HFD mice were less prominent in the [Gly2]-GLP-2 treated group. TUNEL-positive cell number in brain sections of [Gly2]-GLP-2-treated HFD-fed mice was significantly lesser in comparison with untreated-HFD animals and similar to STD fed mice. In conclusion, the results of the present study suggest that GLP-2 stable analogue improves the obesity-associated neuroinflammation and the central stress conditions, it reduces the neuronal apoptotic death, providing evidence for a neuroprotective role of the peptide.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Inflammation
medicine.disease_cause
Diet, High-Fat
Settore BIO/09 - Fisiologia
Neuroprotection
lcsh:RC321-571
03 medical and health sciences
0302 clinical medicine
Neuroinflammation
Internal medicine
medicine
Glucagon-Like Peptide 2
Animals
Obesity
Neurodegeneration
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
TUNEL assay
Glial fibrillary acidic protein
biology
Chemistry
digestive, oral, and skin physiology
Brain
medicine.disease
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
Neuroprotective Agents
Neurology
Gliosis
Oxidative stress
Astrocytes
biology.protein
Glucagon-Like Peptide-2 Receptor
Oxidative stre
Encephalitis
medicine.symptom
Inflammation Mediators
GLP-2
030217 neurology & neurosurgery
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 1095953X
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Neurobiology of disease
- Accession number :
- edsair.doi.dedup.....98af9c10b60d3f641246724221f0096c