Additional file 6: figure S6. of Biochemical and biophysical characterization of cell-free synthesized Rift Valley fever virus nucleoprotein capsids enables in vitro screening to identify novel antivirals

Full conversion of the intermediate capsid structures into highly ordered capsids by nonradioactive CFPS-products. Glycerol-gradient fractionation profiles of the radioactive material generated by CFPS under conditions favoring intermediate-assembly structures and “chased” by the addition of intermediate-assembly structures of nonradioactive products. As indicated: dark blue, “mock” chase experiment (addition of buffer only); green, “chase” experiment (addition of nonradioactive intermediate structures). The chase was performed for 45 min at 37 °C. (PDF 53 kb)