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DISC1, PDE4B, and NDE1 at the centrosome and synapse
- Source :
- Biochemical and Biophysical Research Communications, Biochemical and Biophysical Research Communications, Elsevier, 2008, 377 (4), pp.1091-1096. ⟨10.1016/j.bbrc.2008.10.120⟩
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- International audience; Disrupted-In-Schizophrenia 1 (DISC1) is a risk factor for schizophrenia and other major mental illnesses. Its protein binding partners include the Nuclear Distribution Factor E Homologs (NDE1 and NDEL1), LIS1, and phosphodiesterases 4B and 4D (PDE4B and PDE4D). We demonstrate that NDE1, NDEL1 and LIS1, together with their binding partner dynein, associate with DISC1, PDE4B and PDE4D within the cell, and provide evidence that this complex is present at the centrosome. LIS1 and NDEL1 have been previously suggested to be synaptic, and we now demonstrate localisation of DISC1, NDE1, and PDE4B at syn-apses in cultured neurons. NDE1 is phosphorylated by cAMP-dependant Protein Kinase A (PKA), whose activity is, in turn, regulated by the cAMP hydrolysis activity of phosphodiesterases, including PDE4. We propose that DISC1 acts as an assembly scaffold for all of these proteins and that the NDE1/ NDEL1/LIS1/dynein complex is modulated by cAMP levels via PKA and PDE4.
- Subjects :
- [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Dynein
Biophysics
Nerve Tissue Proteins
Plasma protein binding
Biochemistry
Synapse
03 medical and health sciences
DISC1
0302 clinical medicine
NDEL1
PDE4
LIS1
NDE1
Schizophrenia
Centrosome
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Cyclic AMP
Humans
Phosphorylation
Protein kinase A
Molecular Biology
Cells, Cultured
030304 developmental biology
0303 health sciences
[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]
biology
Phosphodiesterase
Cell Biology
Activating Transcription Factor 4
Cyclic AMP-Dependent Protein Kinases
3. Good health
Cell biology
Cyclic Nucleotide Phosphodiesterases, Type 4
1-Alkyl-2-acetylglycerophosphocholine Esterase
Synapses
biology.protein
Microtubule-Associated Proteins
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X and 10902104
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications, Biochemical and Biophysical Research Communications, Elsevier, 2008, 377 (4), pp.1091-1096. ⟨10.1016/j.bbrc.2008.10.120⟩
- Accession number :
- edsair.doi.dedup.....9847cd0e16f662b6e17beaa2ca0aabda