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Experimental Validation of ARFI Surveillance of Subcutaneous Hemorrhage (ASSH) Using Calibrated Infusions in a Tissue-Mimicking Model and Dogs
- Source :
- Ultrasonic imaging. 38(5)
- Publication Year :
- 2015
-
Abstract
- Acoustic radiation force impulse (ARFI) Surveillance of Subcutaneous Hemorrhage (ASSH) has been previously demonstrated to differentiate bleeding phenotype and responses to therapy in dogs and humans, but to date, the method has lacked experimental validation. This work explores experimental validation of ASSH in a poroelastic tissue-mimic and in vivo in dogs. The experimental design exploits calibrated flow rates and infusion durations of evaporated milk in tofu or heparinized autologous blood in dogs. The validation approach enables controlled comparisons of ASSH-derived bleeding rate (BR) and time to hemostasis (TTH) metrics. In tissue-mimicking experiments, halving the calibrated flow rate yielded ASSH-derived BRs that decreased by 44% to 48%. Furthermore, for calibrated flow durations of 5.0 minutes and 7.0 minutes, average ASSH-derived TTH was 5.2 minutes and 7.0 minutes, respectively, with ASSH predicting the correct TTH in 78% of trials. In dogs undergoing calibrated autologous blood infusion, ASSH measured a 3-minute increase in TTH, corresponding to the same increase in the calibrated flow duration. For a measured 5% decrease in autologous infusion flow rate, ASSH detected a 7% decrease in BR. These tissue-mimicking and in vivo preclinical experimental validation studies suggest the ASSH BR and TTH measures reflect bleeding dynamics.
- Subjects :
- medicine.medical_specialty
Tissue mimicking phantom
Autologous blood
Hemorrhage
030204 cardiovascular system & hematology
Models, Biological
Article
030218 nuclear medicine & medical imaging
03 medical and health sciences
0302 clinical medicine
Dogs
Subcutaneous Tissue
medicine
Animals
Radiology, Nuclear Medicine and imaging
Radiological and Ultrasound Technology
business.industry
Ultrasound
Reproducibility of Results
Experimental validation
Surgery
Disease Models, Animal
Hemostasis
Calibration
Subcutaneous hemorrhage
Elasticity Imaging Techniques
Time to hemostasis
Nuclear medicine
business
Subjects
Details
- ISSN :
- 10960910
- Volume :
- 38
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Ultrasonic imaging
- Accession number :
- edsair.doi.dedup.....981a9f7b7f498edc64373434a3f9586e