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Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans
- Source :
- Moleculargeneral genetics : MGG. 264(1-2)
- Publication Year :
- 2000
-
Abstract
- An ATM-like gene was identified in the genome of Caenorhabditis elegans. The putative product of the gene, termed Ce-atl-1 (C. elegans ATM-like 1) consists of 2514 amino acid residues. The C-terminal sequence, which contains a PI-3 kinase-like domain, showed good homology with the products of the gene MEC1/ESR1 from budding yeast, the rad3+ gene of fission yeast and mammalian ATR (ataxia-telangiectasia and rad3+ related) genes. The results of RNA-mediated interference indicated that the major phenotype associated with repression of Ce-atl-1 was lethality (approximately 50–80%) during early embryogenesis. Among the surviving progeny, males (XO animals) arose at a high frequency (2–30%). In addition, 5% of oocyte chromosomes demonstrated aneuploidy due to a defect in pre-meiotic chromosomal segregation. Gene expression analyses indicated that Ce-atl-1 mRNA was expressed in all larval stages and that its level increased about fivefold in the adult stage. The adult expression level was decreased in the glp-4 mutant, which is defective in germ line proliferation. Ce-atl-1 was strongly expressed in both the mitotic and meiotic cells of adult gonads. In summary, Ce-atl-1 appears to be important for early embryogenesis, and loss of its function results in a defect in chromosome segregation, similar to what has been observed for AT-related proteins.
- Subjects :
- Male
Embryo, Nonmammalian
Mutant
Molecular Sequence Data
Cell Cycle Proteins
Ataxia Telangiectasia Mutated Proteins
Protein Serine-Threonine Kinases
Homology (biology)
Meiosis
RNA interference
Gene expression
Genetics
Animals
Amino Acid Sequence
Cloning, Molecular
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Molecular Biology
Gene
Mitosis
Phylogeny
biology
Sequence Homology, Amino Acid
Tumor Suppressor Proteins
Phosphotransferases
Gene Expression Regulation, Developmental
Proteins
biology.organism_classification
Molecular biology
DNA-Binding Proteins
Larva
Mutation
Female
RNA, Protozoan
Subjects
Details
- ISSN :
- 00268925
- Volume :
- 264
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Moleculargeneral genetics : MGG
- Accession number :
- edsair.doi.dedup.....980599c0f8d8b9454c63af1610780390