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Effect of partial or complete elimination of light-harvesting complexes on the surface electric properties and the functions of cyanobacterial photosynthetic membranes
- Source :
- Physiologia plantarum. 147(2)
- Publication Year :
- 2012
-
Abstract
- Influence of the modification of the cyanobacterial light-harvesting complex [i.e. phycobilisomes (PBS)] on the surface electric properties and the functions of photosynthetic membranes was investigated. We used four PBS mutant strains of Synechocystis sp. PCC6803 as follows: PAL (PBS-less), CK (phycocyanin-less), BE (PSII-PBS-less) and PSI-less/apcE(-) (PSI-less with detached PBS). Modifications of the PBS content lead to changes in the cell morphology and surface electric properties of the thylakoid membranes as well as in their functions, such as photosynthetic oxygen-evolving activity, P700 kinetics and energy transfer between the pigment-protein complexes. Data reveal that the complete elimination of PBS in the PAL mutant causes a slight decrease in the electric dipole moments of the thylakoid membranes, whereas significant perturbations of the surface charges were registered in the membranes without assembled PBS-PSII macrocomplex (BE mutant) or PSI complex (PSI-less mutant). These observations correlate with the detected alterations in the membrane structural organization. Using a polarographic oxygen rate electrode, we showed that the ratio of the fast to the slow oxygen-evolving PSII centers depends on the partial or complete elimination of light-harvesting complexes, as the slow operating PSII centers dominate in the PBS-less mutant and in the mutant with detached PBS.
- Subjects :
- Physiology
Mutant
macromolecular substances
Plant Science
Biology
Cell morphology
Thylakoids
Light-harvesting complex
Genetics
Surface charge
Photosynthesis
P700
Photosystem I Protein Complex
Synechocystis
Photosystem II Protein Complex
Cell Biology
General Medicine
Electrophysiological Phenomena
Oxygen
Crystallography
Microscopy, Electron
Membrane
Thylakoid
Biophysics
Phycobilisome
Oxidation-Reduction
Gene Deletion
Subjects
Details
- ISSN :
- 13993054
- Volume :
- 147
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Physiologia plantarum
- Accession number :
- edsair.doi.dedup.....980339eb46f5868ebe2a627910a7ee0a