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Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using <scp>CRISPR</scp> /Cas9 gene editing
- Source :
- Plant Biotechnology Journal
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Summary The CRISPR/Cas9 nuclease system is a powerful and flexible tool for genome editing, and novel applications of this system are being developed rapidly. Here, we used CRISPR/Cas9 to target the FAD2 gene in Arabidopsis thaliana and in the closely related emerging oil seed plant, Camelina sativa, with the goal of improving seed oil composition. We successfully obtained Camelina seeds in which oleic acid content was increased from 16% to over 50% of the fatty acid composition. These increases were associated with significant decreases in the less desirable polyunsaturated fatty acids, linoleic acid (i.e. a decrease from ~16% to
- Subjects :
- Fatty Acid Desaturases
0106 biological sciences
0301 basic medicine
Linolenic acid
Linoleic acid
Camelina sativa
Arabidopsis
allohexaploid
Plant Science
Polymerase Chain Reaction
01 natural sciences
Polyploidy
03 medical and health sciences
chemistry.chemical_compound
fatty acid composition
Arabidopsis thaliana
CRISPR/Cas9
Germ-Line Mutation
Research Articles
Gene Editing
2. Zero hunger
chemistry.chemical_classification
biology
Fatty Acids
food and beverages
Fatty acid
Plants, Genetically Modified
biology.organism_classification
Camelina
Plant Leaves
Oleic acid
030104 developmental biology
oleic acid
chemistry
Biochemistry
Brassicaceae
Seeds
CRISPR-Cas Systems
Agronomy and Crop Science
RNA, Guide, Kinetoplastida
Research Article
010606 plant biology & botany
Biotechnology
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 14677652 and 14677644
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology Journal
- Accession number :
- edsair.doi.dedup.....9801269b2c29c00a77c275a33b2a3848