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Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine
- Source :
- Chonnam Medical Journal
- Publication Year :
- 2017
-
Abstract
- Purinergic receptors play an important role in regulating gastrointestinal (GI) motility. Interstitial cells of Cajal (ICCs) are pacemaker cells that regulate GI smooth muscle activity. We studied the functional roles of external adenosine 5'-triphosphate (ATP) on pacemaker activity in cultured ICCs from mouse small intestines by using the whole-cell patch clamp technique and intracellular Ca2+ ([Ca2+]i) imaging. External ATP dose-dependently depolarized the resting membrane and produced tonic inward pacemaker currents, and these effects were antagonized by suramin, a purinergic P2 receptor antagonist. ATP-induced effects on pacemaker currents were suppressed by an external Na+-free solution and inhibited by the nonselective cation channel blockers, flufenamic acid and niflumic acid. The removal of external Ca2+ or treatment with thapsigargin (inhibitor of Ca2+ uptake into endoplasmic reticulum) inhibited the ATP-induced effects on pacemaker currents. Spontaneous [Ca2+]i oscillations were enhanced by external ATP. These results suggest that external ATP modulates pacemaker activity by activating nonselective cation channels via external Ca2+ influx and [Ca2+]i release from the endoplasmic reticulum. Thus, it seems that activating the purinergic P2 receptor may modulate GI motility by acting on ICCs in the small intestine.
- Subjects :
- 0301 basic medicine
Pacemaker, Artificial
Thapsigargin
03 medical and health sciences
chemistry.chemical_compound
symbols.namesake
Adenosine Triphosphate
Intestine, Small
Medicine
Patch clamp
business.industry
Endoplasmic reticulum
Purinergic receptor
Niflumic acid
General Engineering
Receptors, Purinergic
Interstitial Cells of Cajal
Adenosine
Cell biology
Interstitial cell of Cajal
030104 developmental biology
chemistry
symbols
Original Article
business
Adenosine triphosphate
medicine.drug
Subjects
Details
- ISSN :
- 22337385
- Volume :
- 54
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Chonnam medical journal
- Accession number :
- edsair.doi.dedup.....97e9bcf6fe090a3cf48ed5576f975755