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Genetic Evidence for a Contribution of EphA:EphrinA Reverse Signaling to Motor Axon Guidance
- Source :
- Journal of Neuroscience; Vol 32, Journal of Neuroscience
- Publication Year :
- 2012
- Publisher :
- Society for Neuroscience, 2012.
-
Abstract
- Repulsive Eph forward signaling from limb-derived ephrins guides the axons of lateral motor column (LMC) motor neurons. LMC axons also express ephrinAs, while their EphA receptors are expressed in the limb mesenchyme.In vitrostudies have suggested that reverse signaling from limb-derived EphA4 to axonal ephrinAs might result in attraction of LMC axons. However, genetic evidence for this function is lacking. Here we use the Dunn chamber turning assay to show that EphA proteins are chemoattractants and elicit fast turning responses in LMC neuronsin vitro. Moreover, ectopic expression of EphA4 in chick hindlimb changes the limb trajectory of LMC axons. Nervous system-specific deletion of EphA4 in mice resulted in fewer LMC axon projection errors than the ubiquitous deletion of EphA4. Additionally, a signaling-incompetent EphA4 mutant partially rescued guidance errors in the hindlimb, suggesting that limb-derived EphA4 contributes to the establishment of LMC projections. In summary, we provide evidence for a role of EphA:ephrinA attractive reverse signaling in motor axon guidance andin vivoevidence of in-parallel forward Eph and reverse ephrin signaling function in the same neuronal population.
- Subjects :
- Mesenchyme
Chick Embryo
Hindlimb
Biology
Efferent Pathways
Mice
03 medical and health sciences
0302 clinical medicine
Cell Movement
medicine
Animals
Ephrin
Axon
Cells, Cultured
Receptors, Eph Family
030304 developmental biology
Mice, Knockout
Motor Neurons
0303 health sciences
General Neuroscience
Erythropoietin-producing hepatocellular (Eph) receptor
Immunohistochemistry
Axons
medicine.anatomical_structure
nervous system
Electrophoresis, Polyacrylamide Gel
Ectopic expression
Axon guidance
Signal transduction
Brief Communications
Ephrins
Neuroscience
030217 neurology & neurosurgery
Signal Transduction
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....97e16c5b7399b58c2c1a4f26375b9156
- Full Text :
- https://doi.org/10.1523/jneurosci.5707-11.2012