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PCR-based identification ofStaphylococcus epidermidistargetinggseAencoding the glutamic-acid-specific protease
- Source :
- Canadian Journal of Microbiology. 50:493-498
- Publication Year :
- 2004
- Publisher :
- Canadian Science Publishing, 2004.
-
Abstract
- The frequency of the gseA gene encoding a glutamic acid-specific serine protease, GluSE, of Staphylococcus epidermidis was investigated. DNA hybridization analysis demonstrated that gseA existed exclusively in S. epidermidis but not in other bacteria examined. A single step PCR assay with a set of designed primers yielded amplification of gseA from all 69 clinical isolates of S. epidermidis taken from patients and healthy adults, whereas production of GluSE was observed in 74% (51/69) of the isolates. Furthermore, none of the 46 clinical isolates of other species of coagulase-negative staphylococci and 45 clinical isolates of Staphylococcus aureus showed amplification, except a Staphylococcus capitis strain. However, this strain was positive for a S. epidermidis-specific DNA region and the DNA sequence of the 16S rRNA gene showed 99% identity with that of S. epidermidis. Therefore, these results indicated that the present PCR assay for gseA was ubiquitous and highly specific for detection of S. epidermidis.Key words: Staphylococcus epidermidis, glutamic acid-specific protease, PCR assay, molecular identification.
- Subjects :
- DNA, Bacterial
Staphylococcus aureus
Staphylococcus
Immunology
Staphylococcal infections
medicine.disease_cause
DNA, Ribosomal
Polymerase Chain Reaction
Sensitivity and Specificity
Applied Microbiology and Biotechnology
Microbiology
law.invention
Bacterial Proteins
law
Staphylococcus epidermidis
RNA, Ribosomal, 16S
Genetics
medicine
Humans
Molecular Biology
Polymerase chain reaction
DNA Primers
Bacteriological Techniques
biology
DNA–DNA hybridization
Serine Endopeptidases
Genes, rRNA
General Medicine
Staphylococcal Infections
medicine.disease
biology.organism_classification
16S ribosomal RNA
Molecular biology
Staphylococcus capitis
Genes, Bacterial
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....97ceede2f9fd61d47f061db8ec7f8c11
- Full Text :
- https://doi.org/10.1139/w04-055