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PCR-based identification ofStaphylococcus epidermidistargetinggseAencoding the glutamic-acid-specific protease

Authors :
K Ishibashi
Y Ikeda
Y Ohara-Nemoto
S Kimura
K Kikuchi
Source :
Canadian Journal of Microbiology. 50:493-498
Publication Year :
2004
Publisher :
Canadian Science Publishing, 2004.

Abstract

The frequency of the gseA gene encoding a glutamic acid-specific serine protease, GluSE, of Staphylococcus epidermidis was investigated. DNA hybridization analysis demonstrated that gseA existed exclusively in S. epidermidis but not in other bacteria examined. A single step PCR assay with a set of designed primers yielded amplification of gseA from all 69 clinical isolates of S. epidermidis taken from patients and healthy adults, whereas production of GluSE was observed in 74% (51/69) of the isolates. Furthermore, none of the 46 clinical isolates of other species of coagulase-negative staphylococci and 45 clinical isolates of Staphylococcus aureus showed amplification, except a Staphylococcus capitis strain. However, this strain was positive for a S. epidermidis-specific DNA region and the DNA sequence of the 16S rRNA gene showed 99% identity with that of S. epidermidis. Therefore, these results indicated that the present PCR assay for gseA was ubiquitous and highly specific for detection of S. epidermidis.Key words: Staphylococcus epidermidis, glutamic acid-specific protease, PCR assay, molecular identification.

Details

ISSN :
14803275 and 00084166
Volume :
50
Database :
OpenAIRE
Journal :
Canadian Journal of Microbiology
Accession number :
edsair.doi.dedup.....97ceede2f9fd61d47f061db8ec7f8c11
Full Text :
https://doi.org/10.1139/w04-055