Back to Search
Start Over
Calcitonin gene‑related peptide induces IL‑6 expression in RAW264.7 macrophages mediated by mmu_circRNA_007893
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2016
-
Abstract
- Several circular RNAs (circRNAs) may have role important roles in biological processes, however, there is limited knowledge of circRNAs and their potential functions in RAW264.7 macrophages. The present study aimed to examine the expression of circRNAs and explore their effects on interleukin‑6 (IL‑6) expression induced by calcitonin gene‑related peptide (CGRP) in RAW264.7 macrophages. To identify circRNAs, the circRNA expression was measured in macrophages with or without CGRP stimulation. The interaction between circRNAs and microRNAs (miRs) were then identified using bioinformatic software and networks. In the current study, it was demonstrated that CGRP increased the expression of IL‑6 mRNA in a dose‑ and time‑dependent manner. Furthermore, mmu_circRNA_007893 was significantly increased in the CGRP‑stimulated macrophages. Silencing of mmu_circRNA_007893, IL‑6 mRNA expression was significantly decreased, whereas mmu‑miR‑485‑5p expression was markedly increased. Furthermore, when overexpression of mmu‑miR‑485‑5p, IL‑6 mRNA was markedly decreased. The results demonstrated that CGRP‑induced IL‑6 mRNA expression was mediated by mmu_circRNA_007893, and mmu_circRNA_007893 functioned as an endogenous mmu‑miR‑485‑5p sponge as part of induction of IL‑6 mRNA expression.
- Subjects :
- 0301 basic medicine
Cancer Research
Calcitonin Gene-Related Peptide
Cell
Calcitonin gene-related peptide
Biology
Biochemistry
03 medical and health sciences
Mice
microRNA
Genetics
medicine
Gene silencing
Animals
RNA, Messenger
Molecular Biology
Messenger RNA
Oncogene
Base Sequence
Interleukin-6
Macrophages
RNA
Reproducibility of Results
circular RNA
RNA, Circular
Articles
Molecular medicine
Molecular biology
Up-Regulation
030104 developmental biology
medicine.anatomical_structure
RAW 264.7 Cells
Oncology
Molecular Medicine
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 16
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....9799fdc0347c3927746ab1b853fbfed8