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Development of a method for assessing the accumulation and metabolization of antidepressant drugs in zebrafish (Danio rerio) eleutheroembryos
- Source :
- Analytical and Bioanalytical Chemistry, E-Prints Complutense. Archivo Institucional de la UCM, instname
- Publication Year :
- 2021
-
Abstract
- Antidepressant drugs are widely used for the treatment of common mental or other psychiatric disorders such as depression, which affect about 121 million people worldwide. This widespread use has contributed to the input of these pharmaceuticals and their metabolites into the environment. The aim of this work was to develop an analytical method to quantify the most widely used antidepressant drugs, selective serotonin reuptake inhibitors (SSRI), and their main metabolites in the environment. For this, a new and reliable miniaturized extraction method based on dispersive SPE cleanup procedure for extraction of SSRI followed by derivatization with n-heptafluorobutyrylimidazole, and detection by GC-MS was developed. The methodology, including a first-order one-compartment model, was then applied to a bioconcentration study in zebrafish (Danio rerio) eleutheroembryos. The results showed low bioaccumulation of these compounds; however, a biotransformation evidence of the parent compounds into their metabolites was observed after 6 h of exposure. These results indicate the need to integrate metabolic transformation rates to fully model and understand the bioaccumulation patterns of SSRI and their metabolites. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03486-2.
- Subjects :
- Embryo, Nonmammalian
SSRIs and metabolites
Danio
Bioconcentration
010501 environmental sciences
Pharmacology
01 natural sciences
Biochemistry
Analytical Chemistry
Biotransformation
Animals
Zebrafish
0105 earth and related environmental sciences
biology
Zebrafish eleutheroembryos
010401 analytical chemistry
Reproducibility of Results
Química
Química analítica
Serotonin reuptake
biology.organism_classification
0104 chemical sciences
Bioaccumulation
Antidepressant
Extraction methods
Environmental Pollutants
GC-MS
Selective Serotonin Reuptake Inhibitors
Environmental Monitoring
Research Paper
Subjects
Details
- ISSN :
- 16182650
- Volume :
- 413
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Analytical and bioanalytical chemistry
- Accession number :
- edsair.doi.dedup.....978969a3a7eff422860b155e135dcf1f