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Phosphatidylserine on viable sperm and phagocytic machinery in oocytes regulate mammalian fertilization
- Source :
- Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019), Nature Communications, NATURE COMMUNICATIONS
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Fertilization is essential for species survival. Although Izumo1 and Juno are critical for initial interaction between gametes, additional molecules necessary for sperm:egg fusion on both the sperm and the oocyte remain to be defined. Here, we show that phosphatidylserine (PtdSer) is exposed on the head region of viable and motile sperm, with PtdSer exposure progressively increasing during sperm transit through the epididymis. Functionally, masking phosphatidylserine on sperm via three different approaches inhibits fertilization. On the oocyte, phosphatidylserine recognition receptors BAI1, CD36, Tim-4, and Mer-TK contribute to fertilization. Further, oocytes lacking the cytoplasmic ELMO1, or functional disruption of RAC1 (both of which signal downstream of BAI1/BAI3), also affect sperm entry into oocytes. Intriguingly, mammalian sperm could fuse with skeletal myoblasts, requiring PtdSer on sperm and BAI1/3, ELMO2, RAC1 in myoblasts. Collectively, these data identify phosphatidylserine on viable sperm and PtdSer recognition receptors on oocytes as key players in sperm:egg fusion.<br />Izumo and Juno are receptors on sperm and eggs respectively required for fusion, but other factors for sperm-egg fusion are poorly studied. Here, the authors report that phosphatidylserine, found mainly on cells marked for death, is also present on motile sperm, recognized by egg receptors, and is required for sperm-egg fusion.
- Subjects :
- 0301 basic medicine
CD36 Antigens
Male
rac1 GTP-Binding Protein
COUPLED RECEPTOR BAI3
AMINOPHOSPHOLIPIDS
Germline development
General Physics and Astronomy
chemistry.chemical_compound
Myoblast fusion
Mice
0302 clinical medicine
Human fertilization
Medicine and Health Sciences
Angiogenic Proteins
Receptor
lcsh:Science
reproductive and urinary physiology
Epididymis
Phagocytes
Multidisciplinary
Phosphatidylserine
Spermatozoa
Cell biology
medicine.anatomical_structure
MYOBLAST FUSION
Models, Animal
embryonic structures
Female
endocrine system
Myoblasts, Skeletal
Science
REQUIREMENT
Nerve Tissue Proteins
Receptors, Cell Surface
Phosphatidylserines
Biology
ENGULFMENT
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
Developmental biology
medicine
Animals
Humans
APOPTOTIC CELLS
EGG
Adaptor Proteins, Signal Transducing
Sperm-Ovum Interactions
c-Mer Tyrosine Kinase
urogenital system
Neuropeptides
Membrane Proteins
Biology and Life Sciences
General Chemistry
CD9
Oocyte
Sperm
Mice, Inbred C57BL
Cytoskeletal Proteins
030104 developmental biology
Sperm entry
chemistry
PLASMA-MEMBRANE
Oocytes
lcsh:Q
CD36
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....977e6af026c9a6ad432f636746f12a12