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Human GLI-2 Is a Tat Activation Response Element-Independent Tat Cofactor
- Source :
- Journal of Virology. 75:2314-2323
- Publication Year :
- 2001
- Publisher :
- American Society for Microbiology, 2001.
-
Abstract
- Zinc finger-containing GLI proteins are involved in the development of Caenorhabditis elegans, Xenopus, Drosophila , zebrafish, mice, and humans. In this study, we show that an isoform of human GLI-2 strongly synergizes with the Tat transactivating proteins of human immunodeficiency virus types 1 and 2 (HIV-1 and -2) and markedly stimulates viral replication. GLI-2 also synergizes with the previously described Tat cofactor cyclin T1 to stimulate Tat function. Surprisingly, GLI-2/Tat synergy is not dependent on either a typical GLI DNA binding site or an intact Tat activation response element but does require an intact TATA box. Thus, GLI-2/Tat synergy results from a mechanism of action which is novel both for a GLI protein and for a Tat cofactor. These findings link the GLI family of transcriptional and developmental regulatory proteins to Tat function and HIV replication.
- Subjects :
- Transcriptional Activation
animal structures
Cyclin T1
TATA box
Immunology
Response element
Kruppel-Like Transcription Factors
Xenopus
HIV Infections
Receptors, Cell Surface
Zinc Finger Protein Gli2
Response Elements
Transfection
Virus Replication
Microbiology
Cell Line
Mice
Bacterial Proteins
Cyclins
Virology
Animals
Drosophila Proteins
Humans
Nuclear protein
Transcription factor
integumentary system
biology
Cyclin T
Escherichia coli Proteins
Membrane Proteins
Nuclear Proteins
biology.organism_classification
TATA Box
Molecular biology
Chemoreceptor Cells
Virus-Cell Interactions
DNA binding site
Membrane protein
Insect Science
Gene Products, tat
HIV-2
embryonic structures
HIV-1
tat Gene Products, Human Immunodeficiency Virus
Plasmids
Transcription Factors
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....9776dbc4b1a5142adb55b0de84558ca8