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Toxicological Evaluation of SiO2 Nanoparticles by Zebrafish Embryo Toxicity Test
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2019, 20 (4), pp.882. ⟨10.3390/ijms20040882⟩, International Journal of Molecular Sciences, Vol 20, Iss 4, p 882 (2019), Volume 20, Issue 4
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- As the use of nanoparticles (NPs) is increasing, the potential toxicity and behavior of NPs in living systems need to be better understood. Our goal was to evaluate the developmental toxicity and bio-distribution of two different sizes of fluorescently-labeled SiO2 NPs, 25 and 115 nm, with neutral surface charge or with different surface functionalization, rendering them positively or negatively charged, in order to predict the effect of NPs in humans. We performed a zebrafish embryo toxicity test (ZFET) by exposing the embryos to SiO2 NPs starting from six hours post fertilization (hpf). Survival rate, hatching time, and gross morphological changes were assessed at 12, 24, 36, 48, 60, and 72 hpf. We evaluated the effect of NPs on angiogenesis by counting the number of sub-intestinal vessels between the second and seventh intersegmental vessels and gene expression analysis of vascular endothelial growth factor (VEGF) and VEGF receptors at 72 hpf. SiO2 NPs did not show any adverse effects on survival rate, hatching time, gross morphology, or physiological angiogenesis. We found that SiO2 NPs were trapped by the chorion up until to the hatching stage. After chemical removal of the chorion (dechorionation), positively surface-charged SiO2 NPs (25 nm) significantly reduced the survival rate of the fish compared to the control group. These results indicate that zebrafish chorion acts as a physical barrier against SiO2 NPs, and removing the chorions in ZFET might be necessary for evaluation of toxicity of NPs.
- Subjects :
- Vascular Endothelial Growth Factor A
0301 basic medicine
Embryo, Nonmammalian
Angiogenesis
[SDV]Life Sciences [q-bio]
Developmental toxicity
silica nanoparticles
010501 environmental sciences
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
bio-distribution
lcsh:QH301-705.5
Zebrafish
Spectroscopy
health care economics and organizations
surface functionalization
biology
Chemistry
Gene Expression Regulation, Developmental
Embryo
Chorion
General Medicine
Silicon Dioxide
Computer Science Applications
Vascular endothelial growth factor
Toxicity
embryonic structures
dechorionation
animal structures
embryo acute toxicity test
education
Neovascularization, Physiologic
Protective Agents
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
vascularization
Suspensions
Toxicity Tests
mental disorders
Animals
Physical and Theoretical Chemistry
Molecular Biology
0105 earth and related environmental sciences
Hatching
Organic Chemistry
technology, industry, and agriculture
zebrafish
biology.organism_classification
Survival Analysis
Receptors, Vascular Endothelial Growth Factor
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Biophysics
Nanoparticles
Surface modification
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, MDPI, 2019, 20 (4), pp.882. ⟨10.3390/ijms20040882⟩, International Journal of Molecular Sciences, Vol 20, Iss 4, p 882 (2019), Volume 20, Issue 4
- Accession number :
- edsair.doi.dedup.....975ceb3e9f34e9e62d3260f44751ba95