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Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 21, Iss 3466, p 3466 (2020), Volume 21, Issue 10, Ludvigsen, M, Thorlacius-Ussing, L, Vorum, H, Moyer, M P, Stender, M T, Thorlacius-Ussing, O & Honoré, B 2020, ' Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells : Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer ', International Journal of Molecular Sciences, vol. 21, no. 10, 3466 . https://doi.org/10.3390/ijms21103466
- Publication Year :
- 2020
-
Abstract
- In the western world, colorectal cancer (CRC) is the third most common cause of cancer-related deaths. Survival is closely related to the stage of cancer at diagnosis striking the clinical need for biomarkers capable of early detection. To search for possible biological parameters for early diagnosis of CRC we evaluated protein expression for three CREC (acronym: Cab45, reticulocalbin, ERC-55, calumenin) proteins: reticulocalbin, calumenin, and ERC-55 in a cellular model consisting of a normal derived colon mucosa cell line, NCM460, and a primary adenocarcinoma cell line of the colon, SW480. Furthermore, this cellular model was analyzed by a top-down proteomic approach, 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and liquid chromatography&ndash<br />tandem mass spectrometry (LC&ndash<br />MS/MS) for novel putative diagnostic markers by identification of differentially expressed proteins between the two cell lines. A different colorectal carcinoma cell line, HCT 116, was used in a bottom-up proteomic approach with label-free quantification (LFQ) LC&ndash<br />MS/MS. The two cellular models gave sets of putative diagnostic CRC biomarkers. Various of these novel putative markers were verified with increased expression in CRC patient neoplastic tissue compared to the expression in a non-involved part of the colon, including reticulocalbin, calumenin, S100A6 and protein SET. Characterization of these novel identified biological features for CRC patients may have diagnostic potential and therapeutic relevance in this malignancy characterized by a still unmet clinical need.
- Subjects :
- Proteomics
Male
Proteome
Colorectal cancer
Cell Cycle Proteins
Colorectal adenocarcinoma
S100 Calcium Binding Protein A6
lcsh:Chemistry
Stage (cooking)
Intestinal Mucosa
lcsh:QH301-705.5
Spectroscopy
Aged, 80 and over
General Medicine
Middle Aged
Computer Science Applications
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
colorectal adenocarcinoma
Cellular model system
Female
Cellular model
Colorectal Neoplasms
colorectal cancer
Malignancy
Catalysis
Article
Inorganic Chemistry
proteomics
medicine
Biomarkers, Tumor
Humans
Histone Chaperones
Physical and Theoretical Chemistry
Molecular Biology
protein expression
Aged
business.industry
Organic Chemistry
Calcium-Binding Proteins
Cancer
biomarkers
medicine.disease
HCT116 Cells
digestive system diseases
lcsh:Biology (General)
lcsh:QD1-999
Cell culture
cellular model system
Cancer research
Protein expression
Reticulocalbin 1
business
Biomarkers
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 21
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....97351fd936f143f461e44043a8ed966b