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High-Density Cell Arrays for Genome-Scale Phenotypic Screening
- Source :
- SLAS discovery : advancing life sciences RD. 24(3)
- Publication Year :
- 2019
-
Abstract
- Due to high associated costs and considerable time investments of cell-based screening, there is a strong demand for new technologies that enable preclinical development and tests of diverse biologicals in a cost-saving and time-efficient manner. For those reasons we developed the high-density cell array (HD-CA) platform, which miniaturizes cell-based screening in the form of preprinted and ready-to-run screening arrays. With the HD-CA technology, up to 24,576 samples can be tested in a single experiment, thereby saving costs and time for microscopy-based screening by 75%. Experiments on the scale of the entire human genome can be addressed in a real parallel manner, with screening campaigns becoming more comfortable and devoid of robotics infrastructure on the user side. The high degree of miniaturization enables working with expensive reagents and rare and difficult-to-obtain cell lines. We have also optimized an automated imaging procedure for HD-CA and demonstrate the applicability of HD-CA to CRISPR-Cas9- and RNAi-mediated phenotypic assessment of the gene function.
- Subjects :
- Computer science
Phenotypic screening
Cytological Techniques
Genome scale
High density
Computational biology
Biochemistry
Analytical Chemistry
570 Life sciences
Cell Line
03 medical and health sciences
0302 clinical medicine
Humans
030304 developmental biology
0303 health sciences
Miniaturization
Cellular array
Epidermal Growth Factor
Genome, Human
Robotics
Endocytosis
Phenotype
030220 oncology & carcinogenesis
Molecular Medicine
Human genome
RNA Interference
CRISPR-Cas Systems
Biotechnology
Subjects
Details
- ISSN :
- 24725560
- Volume :
- 24
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- SLAS discovery : advancing life sciences RD
- Accession number :
- edsair.doi.dedup.....96effce7415e9964a7edf0294ed7e11b