Back to Search
Start Over
Identification of IL-18 and Th17 Cells in Salivary Glands of Patients with Sjögren’s Syndrome, and Amplification of IL-17-Mediated Secretion of Inflammatory Cytokines from Salivary Gland Cells by IL-18
- Source :
- The Journal of Immunology. 181:2898-2906
- Publication Year :
- 2008
- Publisher :
- The American Association of Immunologists, 2008.
-
Abstract
- IL-18 is a proinflammatory cytokine and plays an important pathogenic role in inflammatory and autoimmune disorders. IL-17 is also a proinflammatory cytokine and IL-17-secreting Th17 cells are involved in autoimmunity. However, the pathological roles of IL-18 and Th17 cells in Sjögren’s syndrome (SS) remain to be elucidated. This study showed that the expression of IL-18 was detected in acinar cells, intraducts, and CD68+ macrophages in salivary glands of SS patients, but not in those of healthy subjects or patients with chronic graft-vs-host disease, by immunohistochemistry, and immunoblot analysis revealed that 24-kDa precursor form of IL-18 (proIL-18) and 18-kDa mature IL-18 were detected in SS salivary glands. The majority of the infiltrating cells in the salivary glands of SS patients were CD4+ T cells, and CD8+ T cells were infiltrated to a lesser extent. The predominant expression of IL-17 was found in infiltrating CD4+ T cells, whereas a small number of infiltrating CD8+ T cells expressed IL-17. Human salivary gland HSY and acinar AZA3 cells constitutively expressed proIL-18 and caspase-1, and a calcium ionophore A23187 induced the secretion of IL-18 from the cells. HSY and AZA3 cells expressed IL-18R and IL-17R on the cell surface, and IL-18 amplified the secretion of IL-6 and IL-8 that were induced by low amounts of IL-17. Primary salivary gland cells from normal subjects partially confirmed these findings. These results suggest that IL-18 and Th17 cells detected in the salivary glands in SS patients are associated with the pathogenesis of SS in the salivary glands.
- Subjects :
- Adult
medicine.medical_specialty
Immunology
Biology
Salivary Glands, Minor
Proinflammatory cytokine
Cell Movement
T-Lymphocyte Subsets
Cell Line, Tumor
Internal medicine
medicine
Humans
Immunology and Allergy
Secretion
Cells, Cultured
Salivary gland
CD68
Interleukin-17
Interleukin-18
Sjogren's Syndrome
Endocrinology
medicine.anatomical_structure
Cytokines
CCL28
Interleukin 18
Interleukin 17
Inflammation Mediators
CD8
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 181
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....96d882f0dce96a298bcd6094dcd9fd3c