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A new P450 involved in the furanocoumarin pathway underlies a recent case of convergent evolution

Authors :
M.E. Schranz
Robin van Velzen
Sakihito Kitajima
Alain Hehn
Romain Larbat
Ryosuke Munakata
Cloé Villard
Laboratoire Agronomie et Environnement (LAE)
Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Laboratory of Plant Gene Expression
Research Institute for Sustainable Humanosphere (RISH)
Kyoto University [Kyoto]-Kyoto University [Kyoto]
Kyoto Institute of Technology
Wageningen University and Research [Wageningen] (WUR)
Source :
New Phytologist, 231(5), 1923-1939, New Phytologist, New Phytologist, Wiley, 2021, ⟨10.1111/NPH.17458⟩, New Phytologist 231 (2021) 5
Publication Year :
2021

Abstract

Furanocoumarins are phytoalexins often cited as an example to illustrate the arms race between plants and herbivorous insects. They are distributed in a limited number of phylogenetically distant plant lineages, but synthesized through a similar pathway, which raised the question of a unique or multiple emergence in higher plants. The furanocoumarin pathway was investigated in the fig tree (Ficus carica, Moraceae). Transcriptomic and metabolomic approaches led to the identification of CYP76F112, a cytochrome P450 catalyzing an original reaction. CYP76F112 emergence was inquired using phylogenetics combined with in silico modelling and site-directed mutagenesis. CYP76F112 was found to convert demethylsuberosin into marmesin with a very high affinity. This atypical cyclisation reaction represents a key step within the polyphenol biosynthesis pathway. CYP76F112 evolutionary patterns suggests that the marmesin synthase activity appeared recently in the Moraceae family, through a lineage-specific expansion and diversification. The characterization of CYP76F112 as the first known marmesin synthase opens new prospects for the use of the furanocoumarin pathway. It also supports the multiple acquisition of furanocoumarin in angiosperms by convergent evolution, and opens new perspectives regarding the ability of cytochromes P450 to evolve new functions related to plant adaptation to their environment.

Details

Language :
English
ISSN :
0028646X and 14698137
Database :
OpenAIRE
Journal :
New Phytologist, 231(5), 1923-1939, New Phytologist, New Phytologist, Wiley, 2021, ⟨10.1111/NPH.17458⟩, New Phytologist 231 (2021) 5
Accession number :
edsair.doi.dedup.....96b3ccfa5cd6d3efed645a831285dcec
Full Text :
https://doi.org/10.1111/NPH.17458⟩