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A new P450 involved in the furanocoumarin pathway underlies a recent case of convergent evolution
- Source :
- New Phytologist, 231(5), 1923-1939, New Phytologist, New Phytologist, Wiley, 2021, ⟨10.1111/NPH.17458⟩, New Phytologist 231 (2021) 5
- Publication Year :
- 2021
-
Abstract
- Furanocoumarins are phytoalexins often cited as an example to illustrate the arms race between plants and herbivorous insects. They are distributed in a limited number of phylogenetically distant plant lineages, but synthesized through a similar pathway, which raised the question of a unique or multiple emergence in higher plants. The furanocoumarin pathway was investigated in the fig tree (Ficus carica, Moraceae). Transcriptomic and metabolomic approaches led to the identification of CYP76F112, a cytochrome P450 catalyzing an original reaction. CYP76F112 emergence was inquired using phylogenetics combined with in silico modelling and site-directed mutagenesis. CYP76F112 was found to convert demethylsuberosin into marmesin with a very high affinity. This atypical cyclisation reaction represents a key step within the polyphenol biosynthesis pathway. CYP76F112 evolutionary patterns suggests that the marmesin synthase activity appeared recently in the Moraceae family, through a lineage-specific expansion and diversification. The characterization of CYP76F112 as the first known marmesin synthase opens new prospects for the use of the furanocoumarin pathway. It also supports the multiple acquisition of furanocoumarin in angiosperms by convergent evolution, and opens new perspectives regarding the ability of cytochromes P450 to evolve new functions related to plant adaptation to their environment.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
cytochrome P450
In silico
[SDV]Life Sciences [q-bio]
Plant Science
Biology
01 natural sciences
furanocoumarins
03 medical and health sciences
chemistry.chemical_compound
Furanocoumarin
Metabolomics
Cytochrome P-450 Enzyme System
Phylogenetics
Furocoumarins
Convergent evolution
marmesin synthase
convergent evolution
Ficus carica (fig tree)
Phylogeny
ComputingMilieux_MISCELLANEOUS
Ficus
Marmesin
Moraceae
biology.organism_classification
Biosystematiek
030104 developmental biology
chemistry
Evolutionary biology
Biosystematics
Adaptation
EPS
Oxidation-Reduction
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 0028646X and 14698137
- Database :
- OpenAIRE
- Journal :
- New Phytologist, 231(5), 1923-1939, New Phytologist, New Phytologist, Wiley, 2021, ⟨10.1111/NPH.17458⟩, New Phytologist 231 (2021) 5
- Accession number :
- edsair.doi.dedup.....96b3ccfa5cd6d3efed645a831285dcec
- Full Text :
- https://doi.org/10.1111/NPH.17458⟩