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Regioselective labeling of antibodies through N-terminal transamination
- Source :
- ACS chemical biology. 2(4)
- Publication Year :
- 2007
-
Abstract
- A convenient new method is described for the introduction of ketone groups at the N-termini of antibodies. The reaction occurs in the presence of pyridoxal-5′-phosphate under conditions mild enough to maintain antigen binding function, as confirmed by enzyme-linked immunosorbent assay. Further derivatization of these functional sites was accomplished through oxime formation, yielding well-defined antibody conjugates for a wide range of applications. The ability of the modified antibodies to bind their targets was confirmed via immunodot blot analysis. The generality of this method has been demonstrated on a number of monoclonal and polyclonal antibodies, all with different binding specificities.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Ketone
Transamination
Enzyme-Linked Immunosorbent Assay
Biochemistry
Antibodies
chemistry.chemical_compound
Immunoglobulin Fab Fragments
Mice
Antibody Specificity
Oximes
Animals
Amino Acid Sequence
Derivatization
chemistry.chemical_classification
biology
Antibodies, Monoclonal
General Medicine
Ketones
Oxime
Molecular biology
Blot
chemistry
Polyclonal antibodies
Pyridoxal Phosphate
Monoclonal
biology.protein
Molecular Medicine
Antibody
Subjects
Details
- ISSN :
- 15548937
- Volume :
- 2
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- ACS chemical biology
- Accession number :
- edsair.doi.dedup.....96ad6a1a3f60d4be8d2e2006e284b821