Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia

Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India. Cathranthus roseus também conhecida como pervinca, planta ornamental contém vários valores medicinais, foi encontrada com poucos sintomas de folhas e vassouras de bruxa no local de Shahjahanpur, com incidência de até 8%. A etiologia do fitoplasma foi confirmada através do exame de microscopia eletrônica de varredura. Análises moleculares adicionais por meio de PCR aninhado, análise filogênica e silco RFLP confirmaram a associação do fitoplasma do subgrupo 16SrIX-C em pequenas plantas de folhas e vassouras de bruxa, que é o primeiro relatório da Índia.