Induction of cephalosporinase production by various penicillins in enterobacteriaceae

The inducer activity of seven penicillins for cephalosporinase (CSase) production and their antibacterial activity against CSase-producing strains were studied using clinical isolates of Proteus morganii, P. rettgeri, P. vulgaris, Enterobacter cloacae, and Serratia marcescens. Piperacillin, apalcillin, and methicillin showed rather low inducer activity for CSase production in all strains tested. On the other hand, ampicillin, carbenicillin, and sulbenicillin showed high inducer activity for CSase production. Piperacillin, apalcillin, and ampicillin were less stable to CSases than were carbenicillin, sulbenicillin, and methicillin, but much more stable than benzylpenicillin. In the growing culture of CSase-producing strains, piperacillin and apalcillin were rather stable. Against CSase-producing strains, piperacillin and apalcillin were more active than other penicillins tested.