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Adipose tissue-derived stromal cells stimulated macrophages-endothelial cells interactions promote effective ischemic muscle neovascularization
- Source :
- European Journal of Pharmacology. 883:173354
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Neovascularization, the process of new blood vessels formation in response to hypoxia induced signals, is an essential step during wound healing or ischemia repair. It follows as a cascade of consecutive events leading to new blood vessels formation and their subsequent remodeling to a mature and functional state, enabling tissue regeneration. Any disruption in consecutive stages of neovascularization can lead to chronic wounds or impairment of tissue repair. In the study we try to explain the biological basis of accelerated blood vessels formation in ischemic tissue after adipose tissue-derived stromal cells (ADSCs) administration. Experiments were performed on mouse models of hindlimb ischemia. We have evaluated the level of immune cells (neutrophils, macrophages) infiltration. The novelty of our work was the assessment of bone marrow-derived stem/progenitor cells (BMDCs) infiltration and their contribution to the neovascularization process in ischemic tissue. We have noticed that ADSCs regulated immune response and affected the kinetics and ratio of macrophages population infiltrating ischemic tissue. Our research revealed that ADSCs promoted changes in the morphology of infiltrating macrophages and their tight association with forming blood vessels. We assume that recruited macrophages may take over the role of pericytes and stabilize the new blood vessel or even differentiate into endothelial cells, which in consequence can accelerate vascular formation upon ADSCs administration. Our findings indicate that administration of ADSCs into ischemic muscle influence spatio-temporal distribution of infiltrating cells (macrophages, neutrophils and BMDCs), which are involved in each step of vascular formation, promoting effective ischemic tissue neovascularization.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
Stromal cell
Population
Ischemia
Neovascularization, Physiologic
Adipose tissue
Cell Communication
Mesenchymal Stem Cell Transplantation
Neovascularization
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Progenitor cell
Muscle, Skeletal
education
Cells, Cultured
Pharmacology
education.field_of_study
business.industry
Macrophages
Endothelial Cells
Mesenchymal Stem Cells
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
Kinetics
Phenotype
030104 developmental biology
medicine.anatomical_structure
Adipose Tissue
Cell Transdifferentiation
medicine.symptom
Wound healing
business
030217 neurology & neurosurgery
Signal Transduction
Blood vessel
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 883
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....9690132a8136d4e3ee51d0cb09d82c3f