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Bacterial Growth Inhibition Screen (BGIS): harnessing recombinant protein toxicity for rapid and unbiased interrogation of protein function
- Source :
- FEBS lettersReferences. 595(10)
- Publication Year :
- 2021
-
Abstract
- In two proof-of-concept studies, we established and validated the Bacterial Growth Inhibition Screen (BGIS), which explores recombinant protein toxicity in Escherichia coli as a largely overlooked and alternative means for basic characterization of functional eukaryotic protein domains. By applying BGIS, we identified an unrecognized RNA-interacting domain in the DEK oncoprotein (this study) and successfully combined BGIS with random mutagenesis as a screening tool for loss-of-function mutants of the DNA modulating domain of DEK [1]. Collectively, our findings shed new light on the phenomenon of recombinant protein toxicity in E. coli. Given the easy and rapid implementation and wide applicability, BGIS will extend the repertoire of basic methods for the identification, analysis and unbiased manipulation of proteins.
- Subjects :
- Time Factors
Chromosomal Proteins, Non-Histone
Recombinant Fusion Proteins
Protein domain
Mutant
Biophysics
Mutagenesis (molecular biology technique)
Computational biology
Bacterial growth
Biology
medicine.disease_cause
Biochemistry
law.invention
03 medical and health sciences
chemistry.chemical_compound
Bias
Protein Domains
Structural Biology
law
Loss of Function Mutation
Toxicity Tests
Genetics
medicine
Escherichia coli
Animals
Drosophila Proteins
Humans
Poly-ADP-Ribose Binding Proteins
Molecular Biology
030304 developmental biology
Receptors, Eph Family
Oncogene Proteins
0303 health sciences
Oncogene
030302 biochemistry & molecular biology
RNA-Binding Proteins
Reproducibility of Results
Cell Biology
Peptide Fragments
chemistry
Recombinant DNA
Biocatalysis
RNA
DNA
Subjects
Details
- ISSN :
- 18733468
- Volume :
- 595
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- FEBS lettersReferences
- Accession number :
- edsair.doi.dedup.....96873ed1554cf42ebb4df97fc41b9f30