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Human adipose tissue macrophages display activation of cancer-related pathways.: ATM link obesity and cancer?
- Source :
- Journal of Biological Chemistry; Vol 287, Journal of Biological Chemistry, Journal of Biological Chemistry, 2012, 287 (26), pp.21904-13. ⟨10.1074/jbc.M111.315200⟩, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (26), pp.21904-13. ⟨10.1074/jbc.M111.315200⟩
- Publication Year :
- 2012
- Publisher :
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2012.
-
Abstract
- International audience; Obesity is associated with a significantly increased risk for cancer suggesting that adipose tissue dysfunctions might play a crucial role therein. Macrophages play important roles in adipose tissue as well as in cancers. Here, we studied whether human adipose tissue macrophages (ATM) modulate cancer cell function. Therefore, ATM were isolated and compared with monocyte-derived macrophages (MDM) from the same obese patients. ATM, but not MDM, were found to secrete factors inducing inflammation and lipid accumulation in human T47D and HT-29 cancer cells. Gene expression profile comparison of ATM and MDM revealed overexpression of functional clusters, such as cytokine-cytokine receptor interaction (especially CXC-chemokine) signaling as well as cancer-related pathways, in ATM. Comparison with gene expression profiles of human tumor-associated macrophages showed that ATM, but not MDM resemble tumor-associated macrophages. Indirect co-culture experiments demonstrated that factors secreted by preadipocytes, but not mature adipocytes, confer an ATM-like phenotype to MDM. Finally, the concentrations of ATM-secreted factors related to cancer are elevated in serum of obese subjects. In conclusion, ATM may thus modulate the cancer cell phenotype.
- Subjects :
- obesity
medicine.medical_specialty
Chemokine
Adipose tissue macrophages
Adipose tissue
chemokines
Inflammation
Biology
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Neoplasms
Internal medicine
Adipocytes
medicine
Humans
cancer
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Molecular Biology
Oligonucleotide Array Sequence Analysis
030304 developmental biology
Regulation of gene expression
0303 health sciences
Cancer
Cell Biology
medicine.disease
Immunohistochemistry
macrophages
adipose tissue
Gene Expression Regulation, Neoplastic
Phenotype
Metabolism
Endocrinology
Cell culture
030220 oncology & carcinogenesis
Cancer cell
Disease Progression
Cancer research
biology.protein
medicine.symptom
Azo Compounds
Subjects
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Volume :
- 287
- Issue :
- 26
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....96863cb2232379792ec9721f040ea7b3
- Full Text :
- https://doi.org/10.1074/jbc.m111.315200