Histology of Quercus ilex roots during infection by Phytophthora cinnamomi

[EN] This study aimed to elucidate the infection process of the invasive pathogen Phytophthora cinnamomi on primary and secondary roots of 2-month-old Quercus ilex seedlings. To test if different methods of inoculation lead to different changes in the host caused by the pathogen, the root system of plants was either immersed into a suspension of P. cinnamomi zoospores, or placed in direct contact with agar plugs colonized by P. cinnamomi mycelium. Histology of root sections obtained every 24 h for 10 days revealed similar changes in the structure of cells and tissues of the host irrespective of the inoculation method used. However, the immersion method resulted in a delay in the colonization of the host, different aerial symptoms, and the formation of different reproductive structures of the pathogen. Emerging secondary and tertiary roots and sites where secondary or tertiary roots were about to emerge were identified as main entry points. Hyphae in the xylem tissues were more frequently found in secondary than in primary roots, but in both types of roots the phloem was the most important pathway of colonization. For the first time in the interaction between Q. ilex and P. cinnamomi, transmission electron microscopy was used to describe degradation of the host cell walls, pit penetration and extrahaustorial matrix. Haustoria development during intracellular growth and hyphal aggregations (stromata) caused no damage to the host cell walls indicating hemibiotrophic parasitism
This research was financially supported by the Project AGL2011-30438, by the Vicerrectorado de Investigacion from the Polytechnic University of Valencia, and by the "Julio Iranzo'' laboratory from the Botanic Garden of Valencia. The authors deeply appreciate the help of the staff from both institutions, particularly the valuable contribution of Nuria Cebrian Gomez. Additionally, the staff from the microscopy sections from Polytechnic University of Valencia and University of Valencia have provided us with valuable help. We are grateful to the two anonymous reviewers for the valuable comments in an earlier version of this manuscript.