Back to Search
Start Over
MicroRNA profiling of the intestinal tissue of Kazakh sheep after experimental Echinococcus granulosus infection, using a high-throughput approach
- Source :
- Parasite, Parasite, Vol 23, p 23 (2016)
- Publication Year :
- 2016
- Publisher :
- EDP Sciences, 2016.
-
Abstract
- Cystic echinococcosis (CE), caused by infection with the larval stage of the cestode Echinococcus granulosus , is a chronic zoonosis, to which sheep are highly susceptible. Previously, we found that Kazakh sheep with different MHC haplotypes differed in CE infection. Sheep with haplotype MHCMva Ibc-Sac IIab-Hin 1Iab were resistant to CE infection, while their counterparts without this haplotype were not. MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at the post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. To identify microRNA controlling resistance to CE in the early stage of infection, microRNA profiling was conducted in the intestinal tissue of sheep with resistant and non-resistant MHC haplotypes after peroral infection with E. granulosus eggs. A total of 351 known and 186 novel miRNAs were detected in the resistant group, against 353 known and 129 novel miRNAs in the non-resistant group. Among these miRNAs, 83 known miRNAs were significantly differentially expressed, including 75 up-regulated and 8 down-regulated miRNAs. Among these known microRNAs, miR-21-3p, miR-542-5p, miR-671, miR-134-5p, miR-26b, and miR-27a showed a significantly higher expression in CE-resistant sheep compared to the CE-non-resistant library, with the FC > 3. Functional analysis showed that they were NF-kB pathway-responsive miRNAs, which are involved in the inflammation process. The results suggest that these microRNAs may play important roles in the response of intestinal tissue to E. granulosus .
- Subjects :
- 0301 basic medicine
Sequence analysis
Veterinary (miscellaneous)
030231 tropical medicine
Sheep Diseases
Kazakh
Major histocompatibility complex
MHC haplotype
lcsh:Infectious and parasitic diseases
Major Histocompatibility Complex
03 medical and health sciences
0302 clinical medicine
Echinococcosis
microRNA
Gene expression
medicine
Cystic Echinococcosis
Animals
lcsh:RC109-216
Echinococcus granulosus
Disease Resistance
Genetics
Sheep
biology
Sequence Analysis, RNA
Haplotype
Zoonosis
High-Throughput Nucleotide Sequencing
medicine.disease
biology.organism_classification
language.human_language
Echinococcus
Intestines
MicroRNAs
030104 developmental biology
Infectious Diseases
Haplotypes
Insect Science
biology.protein
language
Animal Science and Zoology
Parasitology
Female
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17761042 and 1252607X
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Parasite
- Accession number :
- edsair.doi.dedup.....9679f30a06dc9d1df2e579a728dcd3fd