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Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa (Medicago sativa L.) cultivars in response to aphid feeding
- Source :
- Journal of Integrative Agriculture, Vol 17, Iss 11, Pp 2518-2527 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Plant breeding for resistance to agricultural pests is an essential element in the development of integrated crop management systems, however, the molecular and genetic mechanisms underlying resistance are poorly understood. In this pilot study, we conducted a transcriptomic analysis of a resistant (R) and susceptible (S) variety of alfalfa, with (+A) or without (–A) aphids (totally four treatments). We used the resistant cultivar Zhongmu 1 and the susceptible cultivar Soca. A total of 3 549 mRNAs were differentially expressed, of which 1 738 up-regulated and 1 307 down-regulated genes were identified in S+A/S–A plants, while 543 up-regulated and 331 down-regulated genes were identified in the R+A/R–A plants. KEGG analysis mapped 112 and 546 differentially expressed genes to 8 and 17 substantially enriched pathways for Zhongmu 1 and Soca, respectively. Six shared pathways were linked to plant resistance traits, including phenylpropanoid biosynthesis associated with salicylic acid synthesis, and linoleic acid metabolism associated with both jasmonic acid and flavonoid biosynthesis. Ultimately, we proposed a preliminary regulatory mechanism of alfalfa cultivar resistance response to aphids feeding based on transcriptome analyses and published documents.
- Subjects :
- 0301 basic medicine
alfalfa cultivar resistance
Agriculture (General)
Plant Science
Biology
Biochemistry
S1-972
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
Food Animals
Botany
Cultivar
Plant breeding
KEGG
Ecology
Phenylpropanoid
Jasmonic acid
fungi
food and beverages
transcriptomic
aphids
030104 developmental biology
Flavonoid biosynthesis
chemistry
Animal Science and Zoology
molecular mechanism
Agronomy and Crop Science
Salicylic acid
Food Science
Subjects
Details
- ISSN :
- 20953119
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Agriculture
- Accession number :
- edsair.doi.dedup.....9652d060eb55e6c4ca7a37779e8d0c1c