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Phasor–FLIM as a Screening Tool for the Differential Diagnosis of Actinic Keratosis, Bowen’s Disease, and Basal Cell Carcinoma
- Source :
- Analytical Chemistry. 89:8104-8111
- Publication Year :
- 2017
- Publisher :
- American Chemical Society (ACS), 2017.
-
Abstract
- The aim of this study was to distinguish basal cell carcinoma (BCC) from actinic keratosis (AK) and Bowen’s disease (BD) by fluorescence lifetimes of hematoxylin and eosin (H&E) and phasor analysis. Pseudocolor images of average fluorescence lifetime (τm) exhibited more contrast than conventional bright field and/or fluorescence images of H&E-stained sections. The mean values (μ) of τm distribution (τmμ) in three layers of skin were first explored for comparison with the corresponding layers of AK, BD, and BCC. Moreover, analysis of the H&E fluorescence lifetimes in the phasor space was performed by observing clusters in specific regions of the phasor plot. Various structures in the skin were distinguished. Comparisons of phase distributions from the corresponding layers of skin resulted in quantitative separation and calculation of distinctive parameters including coordinate values, diagonal slopes, and phasor areas. The combination of fluorescence lifetime imaging microscopy (FLIM) and phasor approach (...
- Subjects :
- 0301 basic medicine
Fluorescence-lifetime imaging microscopy
Skin Neoplasms
Keratosis
Analytical chemistry
Bowen's Disease
Analytical Chemistry
Diagnosis, Differential
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
Microscopy
medicine
Humans
Basal cell carcinoma
Bowen's disease
integumentary system
Chemistry
Actinic keratosis
Phasor
medicine.disease
Fluorescence
Keratosis, Actinic
030104 developmental biology
Microscopy, Fluorescence
ROC Curve
Carcinoma, Basal Cell
Area Under Curve
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....964fc87413b004f1e7c2a4256c720988