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Purification, characterization and immunomodulatory activity of a novel polysaccharide from Grifola frondosa

Authors :
Guang-Ying Chen
Weijie Zhang
Yao Chen
Fenmin Zhang
Ting Zhao
Guanghua Mao
Liuqing Yang
Xiangyang Wu
Qian Li
Min Zhang
Source :
International journal of biological macromolecules. 111
Publication Year :
2017

Abstract

A novel bioactive polysaccharide, GFP-22, was isolated from the fruit bodies of Grifola frondosa by anion-exchange and gel filtration chromatography. Structure of GFP-22 was investigated using gas chromatography–mass spectrometry (GC–MS), methylation, nuclear magnetic resonance (NMR), high-performance size exclusion chromatography-multi-angle laser light scattering-refractive index detector (HPSEC-MALLS-RI) and atomic force microscopy (AFM) analysis. The backbone of GFP-22 is composed of 1,4-β- d -Glcp, 1,3-β- d -Glcp, 1,6-α- d -Glcp, 1,6-α- d -Galp, 1,4,6-α- d -Manp and 1,3,6-α- d -Manp units. Molecular weight of GFP-22 is 2.72 × 104 Da. GFP-22 has a linear filamentous structure. The administration of GFP-22 could improve or reverse the CTX-induced immunosuppression, significantly enhance the spleen and thymus indices, spleen lymphocyte proliferation and cytokines production in splenocytes. These findings suggest that GFP-22 could be explored as a natural and potential immunomodulatory agent and as an alternative means of lessening chemotherapy-induced immunosuppression.

Details

ISSN :
18790003
Volume :
111
Database :
OpenAIRE
Journal :
International journal of biological macromolecules
Accession number :
edsair.doi.dedup.....9648a1a24c359810901337d330efe2ca