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Purification, characterization and immunomodulatory activity of a novel polysaccharide from Grifola frondosa
- Source :
- International journal of biological macromolecules. 111
- Publication Year :
- 2017
-
Abstract
- A novel bioactive polysaccharide, GFP-22, was isolated from the fruit bodies of Grifola frondosa by anion-exchange and gel filtration chromatography. Structure of GFP-22 was investigated using gas chromatography–mass spectrometry (GC–MS), methylation, nuclear magnetic resonance (NMR), high-performance size exclusion chromatography-multi-angle laser light scattering-refractive index detector (HPSEC-MALLS-RI) and atomic force microscopy (AFM) analysis. The backbone of GFP-22 is composed of 1,4-β- d -Glcp, 1,3-β- d -Glcp, 1,6-α- d -Glcp, 1,6-α- d -Galp, 1,4,6-α- d -Manp and 1,3,6-α- d -Manp units. Molecular weight of GFP-22 is 2.72 × 104 Da. GFP-22 has a linear filamentous structure. The administration of GFP-22 could improve or reverse the CTX-induced immunosuppression, significantly enhance the spleen and thymus indices, spleen lymphocyte proliferation and cytokines production in splenocytes. These findings suggest that GFP-22 could be explored as a natural and potential immunomodulatory agent and as an alternative means of lessening chemotherapy-induced immunosuppression.
- Subjects :
- Magnetic Resonance Spectroscopy
Size-exclusion chromatography
Spleen
02 engineering and technology
Lymphocyte proliferation
Mass spectrometry
Polysaccharide
01 natural sciences
Biochemistry
Mice
Structural Biology
Polysaccharides
medicine
Splenocyte
Dietary Carbohydrates
Animals
Humans
Immunologic Factors
Molecular Biology
Grifola frondosa
Cell Proliferation
chemistry.chemical_classification
010405 organic chemistry
Chemistry
General Medicine
Methylation
021001 nanoscience & nanotechnology
0104 chemical sciences
Molecular Weight
medicine.anatomical_structure
RAW 264.7 Cells
Chromatography, Gel
Cytokines
0210 nano-technology
Grifola
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....9648a1a24c359810901337d330efe2ca