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Tumorigenicity of chloral hydrate, trichloroacetic acid, trichloroethanol, malondialdehyde, 4-hydroxy-2-nonenal, crotonaldehyde, and acrolein in the B6C3F1 neonatal mouse
- Source :
- Cancer Letters. 185:13-19
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- The tumorigenicity of chloral hydrate (CH), trichloroacetic acid (TCA), trichloroethanol (TCE), malondialdehyde (MDA), crotonaldehyde, acrolein, and 4-hydroxy-2-nonenal (HNE) was tested in the B6C3F(1) neonatal mouse. Mice were administered i.p. injections of CH (1000, 2000, 2500, and 5000 nmol per animal), TCA (1000 and 2000 nmol), TCE (1000 and 2000 nmol), MDA (1500 and 3000 nmol), crotonaldehyde (1500 and 3000 nmol), acrolein (75 and 150 nmol), and HNE (750 and 1500 nmol) at 8 and 15 days of age. At 12 months, only male mice treated with the positive control chemicals, 4-aminobiphenyl (500 and 1000 nmol) and benzo[a]pyrene (150 and 300 nmol), had incidences of tumors in the liver significantly higher than the solvent control. Additional male mice were dosed as described above and their livers were excised at 24, 48 h, and 7 days after the final dose. Liver DNA was isolated and analyzed by 32P-postlabeling/high-performance liquid chromatography (HPLC) and HPLC/electrochemical detection for MDA-derived adduct (M(1)G) and 8-oxo-2'-deoxyguanosine (8-OHdG) formation, respectively. At 24 and 48 h after the final dose, CH- and TCA-treated mice exhibited significantly higher M(1)G levels than the controls. 8-OHdG formation was also induced by CH, TCA, and MDA. These results suggest that under these experimental conditions the B6C3F(1) neonatal mouse is not sensitive to carcinogens that induce an increase in endogenous DNA adduct formation through lipid peroxidation or oxidative stress.
- Subjects :
- Male
Cancer Research
Carcinogenicity Tests
Chloral hydrate
Ethylene Chlorohydrin
Lipid peroxidation
DNA Adducts
Mice
chemistry.chemical_compound
Liver Neoplasms, Experimental
Malondialdehyde
Electrochemistry
medicine
Animals
Chloral Hydrate
Acrolein
Trichloroacetic Acid
Trichloroacetic acid
Crotonaldehyde
Chromatography, High Pressure Liquid
Crosses, Genetic
Carcinogen
Aldehydes
Mice, Inbred C3H
DNA
Molecular biology
Mice, Inbred C57BL
Animals, Newborn
Liver
Oncology
Biochemistry
chemistry
Toxicity
Carcinogens
Microsomes, Liver
Female
Lipid Peroxidation
Phosphorus Radioisotopes
medicine.drug
Subjects
Details
- ISSN :
- 03043835
- Volume :
- 185
- Database :
- OpenAIRE
- Journal :
- Cancer Letters
- Accession number :
- edsair.doi.dedup.....9621af0c3262bc0afda460332d77615e