Back to Search
Start Over
Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects
- Source :
- Journal of applied microbiology. 105(3)
- Publication Year :
- 2008
-
Abstract
- Aim: To investigate the effect of native, heated and glycated bovine serum albumin (BSA) on the ulcerative colitis (UC) and non-UC colonic microbiota in vitro. Methods and Results: Continuous flow culture (CFC) models of the human colonic microbiota inoculated with faeces from UC and non-UC volunteers were maintained on BSA as growth substrate. Changes in bacterial populations and short-chain fatty acids were determined. UC and non-UC microbiota differed significantly in microbial populations, with elevated numbers of sulfate-reducing bacteria (SRB) and clostridia in the microbiota from UC patients. Compared with native BSA, glycated BSA modulated the gut microbiota of UC patients in vitro towards a more detrimental community structure with significant increases in putatively harmful bacteria (clostridia, bacteroides and SRB; P < 0·009) and decreases in dominant and putatively beneficial bacterial groups (eubacteria and bifidobacteria; P < 0·0004). The levels of beneficial short-chain fatty acids were significantly decreased by heated or glycated BSA, but were increased significantly by native BSA. Conclusion: The UC colonic microbiota maintained in CFC was significantly modified by glycated BSA. Significance and Impact of the Study: Results suggest that dietary glycated protein may impact upon the composition and activity of the colonic microbiota, an important environmental variable in UC.
- Subjects :
- Adult
Glycation End Products, Advanced
Male
Hot Temperature
Colon
Serum albumin
Colony Count, Microbial
Gut flora
Applied Microbiology and Biotechnology
Ribotyping
Microbiology
Clostridia
Feces
fluids and secretions
medicine
Animals
Bacteroides
Humans
Glycated Serum Albumin
Colitis
In Situ Hybridization, Fluorescence
Serum Albumin
Aged
Clostridium
Analysis of Variance
Bacteriological Techniques
biology
Bacteria
Sulfates
Serum Albumin, Bovine
General Medicine
Middle Aged
biology.organism_classification
medicine.disease
Fatty Acids, Volatile
Ulcerative colitis
Food
Case-Control Studies
biology.protein
Cattle
Colitis, Ulcerative
Digestion
Female
Dietary Proteins
Biotechnology
Subjects
Details
- ISSN :
- 13652672
- Volume :
- 105
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of applied microbiology
- Accession number :
- edsair.doi.dedup.....96040e8091130a60164cd35bce410c54