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Hyperspectral imaging in the spatial frequency domain with a supercontinuum source
- Source :
- Journal of biomedical optics, vol 24, iss 7, Journal of Biomedical Optics
- Publication Year :
- 2019
- Publisher :
- Linköpings universitet, Avdelningen för medicinsk teknik, 2019.
-
Abstract
- We introduce a method for quantitative hyperspectral optical imaging in the spatial frequency domain (hs-SFDI) to image tissue absorption (μa) and reduced scattering (μs') parameters over a broad spectral range. The hs-SFDI utilizes principles of spatial scanning of the spectrally dispersed output of a supercontinuum laser that is sinusoidally projected onto the tissue using a digital micromirror device. A scientific complementary metal-oxide-semiconductor camera is used for capturing images that are demodulated and analyzed using SFDI computational models. The hs-SFDI performance is validated using tissue-simulating phantoms over a range of μa and μs' values. Quantitative hs-SFDI images are obtained from an ex-vivo beef sample to spatially resolve concentrations of oxy-, deoxy-, and met-hemoglobin, as well as water and fat fractions. Our results demonstrate that the hs-SFDI can quantitatively image tissue optical properties with 1000 spectral bins in the 580- to 950-nm range over a wide, scalable field of view. With an average accuracy of 6.7% and 12.3% in μa and μs', respectively, compared to conventional methods, hs-SFDI offers a promising approach for quantitative hyperspectral tissue optical imaging. Funding agencies: National Institute of Health (NIH) NIBIB Biomedical Technology Research Center LAMMP [P41EB015890]; NIH [R21EB020953]; Military Medical Photonics Program: AFOSR [FA9550-17-1-0193]; Arnold and Mabel Beckman Foundation
- Subjects :
- Image Processing
Field of view
Optical Physics
01 natural sciences
Phantoms
Digital micromirror device
law.invention
Imaging
Computer-Assisted
law
Models
Image Processing, Computer-Assisted
tissue optical properties
Absorption (electromagnetic radiation)
Coloring Agents
Phantoms, Imaging
Optical Imaging
Hyperspectral imaging
Skeletal
Equipment Design
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
supercontinuum laser
Muscle
Spatial frequency
Algorithms
Radiology, Nuclear Medicine and Medical Imaging
Paper
Materials science
Biomedical Engineering
Bioengineering
Models, Biological
Domain (software engineering)
010309 optics
Biomaterials
Optics
Opthalmology and Optometry
0103 physical sciences
Animals
Muscle, Skeletal
Scattering
business.industry
Lasers
Biological
Supercontinuum
hyperspectral
spatial frequency domain imaging
Special Section on Spatial Frequency Domain Imaging
Cattle
Radiologi och bildbehandling
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of biomedical optics, vol 24, iss 7, Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....95f720a8e2855364e935224902706c9b