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Detection and linkage to mobile genetic elements of tetracycline resistance gene tet(M) in Escherichia coli isolates from pigs
- Source :
- BMC Veterinary Research, Jurado-Rabadan, S, de la Fuente, R, Ruiz-Santa-Quiteria, J A, Orden, J A, de Vries, L E & Agersø, Y 2014, ' Detection and linkage to mobile genetic elements of tetracycline resistance gene tet(M) in Escherichia coli isolates from pigs ', B M C Veterinary Research, vol. 10, no. 1 . https://doi.org/10.1186/1746-6148-10-155
- Publication Year :
- 2013
-
Abstract
- Background: In Escherichia coli the genes involved in the acquisition of tetracycline resistance are mainly tet(A) and tet(B). In addition, tet(M) is the most common tetracycline resistance determinant in enterococci and it is associated with conjugative transposons and plasmids. Although tet(M) has been identified in E. coli, to our knowledge, there are no previous reports studying the linkage of the tet(M) gene in E. coli to different mobile genetic elements. The aim of this study was to determine the occurrence of tet(A), tet(B), and tet(M) genes in doxycycline-resistant E. coli isolates from pigs, as well as the detection of mobile genetic elements linked to tet(M) in E. coli and its possible transfer from enterococci. Results: tet(A) was the most frequently detected gene (87.9%) in doxycycline-resistant isolates. tet(M) was found in 13.1% E. coli isolates. The tet(M) gene was detected in relation with conjugative transposons in 10 out of 36 enterococci isolates analyzed but not in any of E. coli isolates positive for tet(M). Southern blot showed that in E. coli and in most of the enterococci isolates the tet(M) gene was carried on a plasmid. According to the phylogenetic analysis, E. coli contained a new tet(M) allele grouping separately. Mating experiments revealed that tet(M) was carried on a mobile element successfully transferred between enterococci and between enterococci and E. coli. Conclusions: The detection of tet(M) in E. coli isolates from pigs was higher than expected. In our study, tet(M) detected in E. coli seems not to have been transferred from enterococci, although it can not be ruled out that the horizontal transfer of this gene occurred from other intestinal tract bacteria.
- Subjects :
- Transposable element
Tetracycline
Genetic Linkage
Swine
DIVERSITY
ENTEROCOCCUS-FAECALIS
DETERMINANTS
Biology
medicine.disease_cause
Enterococcus faecalis
Microbiology
Plasmid
TRANSPOSONS
stomatognathic system
Bacterial Proteins
Drug Resistance, Bacterial
medicine
polycyclic compounds
Escherichia coli
Animals
Escherichia coli Infections
Southern blot
Swine Diseases
ANTIMICROBIAL RESISTANCE
General Veterinary
IDENTIFICATION
tet(M)
STRAINS
E. coli
HUMANS
General Medicine
Gene Expression Regulation, Bacterial
Horizontal gene transfer
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
veterinary(all)
FOOD-ANIMALS
PREVALENCE
Anti-Bacterial Agents
Interspersed Repetitive Sequences
Pigs
VETERINARY
Mobile genetic elements
medicine.drug
Research Article
Subjects
Details
- ISSN :
- 17466148
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- BMC veterinary research
- Accession number :
- edsair.doi.dedup.....95f64a17c5d46fa1baf7d83468792768
- Full Text :
- https://doi.org/10.1186/1746-6148-10-155