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RPTPε promotes M2-polarized macrophage migration through ROCK2 signaling and podosome formation
- Source :
- Journal of Cell Science. 133
- Publication Year :
- 2020
- Publisher :
- The Company of Biologists, 2020.
-
Abstract
- Cysteinyl-leukotrienes (cys-LTs) have well-characterized physiopathological roles in the development of inflammatory diseases. We have previously found that protein tyrosine phosphatase ε (PTPε) is a signaling partner of CysLT1R, a high affinity receptor for leukotriene D4 (LTD4). There are two major isoforms of PTPε, receptor-like (RPTPε) and cytoplasmic (cyt-)PTPε, both of which are encoded by the PTPRE gene but from different promoters. In most cells, their expression is mutually exclusive, except in human primary monocytes, which express both isoforms. Here, we show differential PTPε isoform expression patterns between monocytes, M1 and M2 human monocyte-derived macrophages (hMDMs), with the expression of glycosylated forms of RPTPε predominantly in M2-polarized hMDMs. Using PTPε-specific siRNAs and expression of RPTPε and cyt-PTPε, we found that RPTPε is involved in monocyte adhesion and migration of M2-polarized hMDMs in response to LTD4. Altered organization of podosomes and higher phosphorylation of the inhibitory Y-722 residue of ROCK2 was also found in PTPε-siRNA-transfected cells. In conclusion, we show that differentiation and polarization of monocytes into M2-polarized hMDMs modulates the expression of PTPε isoforms and RPTPε is involved in podosome distribution, ROCK2 activation and migration in response to LTD4.
- Subjects :
- Gene isoform
rho-Associated Kinases
0303 health sciences
Small interfering RNA
Podosome
Macrophages
Cell Biology
Protein tyrosine phosphatase
Biology
Cell biology
03 medical and health sciences
0302 clinical medicine
Podosomes
Humans
Phosphorylation
Macrophage
ROCK2
Protein Tyrosine Phosphatases
Receptor
030217 neurology & neurosurgery
Signal Transduction
030304 developmental biology
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....95f1b96b314d231ee8a906939a2a7499