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Developing a Drug Screening Platform: MALDI-Mass Spectrometry Imaging of Paper-Based Cultures
- Source :
- Anal Chem
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- Many potential chemotherapeutics fail to reach patients. One of the key reasons is that compounds are tested during the drug discovery stage in two-dimensional (2D) cell cultures, which are often unable to accurately model in vivo outcomes. Three-dimensional (3D) in vitro tumor models are more predictive of chemotherapeutic effectiveness than 2D cultures, and thus, their implementation during the drug screening stage has the potential to more accurately evaluate compounds earlier, saving both time and money. Paper-based cultures (PBCs) are an emerging 3D culture platform in which cells suspended in Matrigel are seeded into paper scaffolds and cultured to generate a tissue-like environment. In this study, we demonstrate the potential of matrix-assisted laser desorption/ionization-mass spectrometry imaging with PBCs (MALDI-MSI-PBC) as a drug screening platform. This method discriminated regions of the PBCs with and without cells and/or drugs, indicating that coupling PBCs with MALDI-MSI has the potential to develop rapid, large-scale, and parallel mass spectrometric drug screens.
- Subjects :
- Paper
Drug
Matrigel
Chemistry
Drug discovery
media_common.quotation_subject
010401 analytical chemistry
Cell Culture Techniques
Drug Evaluation, Preclinical
Antineoplastic Agents
Computational biology
Paper based
HCT116 Cells
010402 general chemistry
Mass spectrometry
01 natural sciences
Article
Mass spectrometry imaging
0104 chemical sciences
Analytical Chemistry
Matrix-assisted laser desorption/ionization
In vivo
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Humans
media_common
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....95d0f4c93713d0bbfa73f46477c183e8