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Hybrid Polycaprolactone/Alginate Scaffolds Functionalized with VEGF to Promote de Novo Vessel Formation for the Transplantation of Islets of Langerhans
- Source :
- Advanced healthcare materials, 5(13), 1606-1616. Wiley, Advanced Healthcare Materials, 5(13), 1606-1616, Advanced healthcare materials, 5(13), 1606-16. John Wiley and Sons Ltd, Advanced Healthcare Materials, 5(13), 1606-1616. Wiley
- Publication Year :
- 2016
-
Abstract
- Although regarded as a promising treatment for type 1 diabetes, clinical islet transplantation in the portal vein is still hindered by a low transplantation outcome. Alternative transplantation sites have been proposed, but the survival of extra-hepatically transplanted islets of Langerhans critically depends on quick revascularization after engraftment. This study aims at developing a new 3D scaffold platform that can actively boost vascularization and may find an application for extra-hepatic islet transplantation. The construct consists of a 3D ring-shaped polycaprolactone (PCL) scaffold with heparinized surface to electrostatically bind vascular endothelial growth factor (VEGF), surrounding a hydrogel core for islets encapsulation. Heparin immobilization improves the amount of VEGF retained by the construct, up to 3.6 fold, compared to untreated PCL scaffolds. In a chicken chorioallanthoic membrane model, VEGF immobilized on the construct enhances angiogenesis in close proximity and on the surface of the scaffolds. After 7 days, islets encapsulated in the alginate core show functional response to glucose stimuli comparable to free-floating islets. Thus, the developed platform has the potential to support rapid vascularization and islet endocrine function.
- Subjects :
- Vascular Endothelial Growth Factor A
0301 basic medicine
Scaffold
endocrine system
Materials science
Alginates
Angiogenesis
Polyesters
Islets of Langerhans Transplantation
Biomedical Engineering
Neovascularization, Physiologic
Pharmaceutical Science
Chick Embryo
02 engineering and technology
Biomaterials
Islets of Langerhans
03 medical and health sciences
chemistry.chemical_compound
Glucuronic Acid
medicine
Animals
Humans
METIS-316964
geography
geography.geographical_feature_category
Tissue Scaffolds
Heparin
Hexuronic Acids
021001 nanoscience & nanotechnology
Islet
22/4 OA procedure
Cell biology
Transplantation
Vascular endothelial growth factor
Vascular endothelial growth factor A
IR-100461
030104 developmental biology
chemistry
Polycaprolactone
0210 nano-technology
Biomedical engineering
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 21922640 and 16061616
- Database :
- OpenAIRE
- Journal :
- Advanced healthcare materials, 5(13), 1606-1616. Wiley, Advanced Healthcare Materials, 5(13), 1606-1616, Advanced healthcare materials, 5(13), 1606-16. John Wiley and Sons Ltd, Advanced Healthcare Materials, 5(13), 1606-1616. Wiley
- Accession number :
- edsair.doi.dedup.....95c68757888cac2316874f60c6edd0f8