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The role of myosin 1c and myosin 1b for surfactant exocytosis
- Source :
- Journal of Cell Science
- Publication Year :
- 2016
- Publisher :
- The Company of Biologists, 2016.
-
Abstract
- Actin and actin-associated proteins have a pivotal effect on regulated exocytosis in secretory cells and influence pre-fusion as well as post-fusion stages of exocytosis. Actin polymerization on secretory granules during the post-fusion phase (formation of an actin coat) is especially important in cells with large secretory vesicles or poorly soluble secretions. Alveolar type II (ATII) cells secrete hydrophobic lipo-protein surfactant, which does not easily diffuse from fused vesicles. Previous work showed that compression of actin coat is necessary for surfactant extrusion. Here, we investigate the role of class 1 myosins as possible linkers between actin and membranes during exocytosis. Live-cell microscopy showed translocation of fluorescently labeled myosin 1b and myosin 1c to the secretory vesicle membrane after fusion. Myosin 1c translocation was dependent on its pleckstrin homology domain. Expression of myosin 1b and myosin 1c constructs influenced vesicle compression rate, whereas only the inhibition of myosin 1c reduced exocytosis. These findings suggest that class 1 myosins participate in several stages of ATII cell exocytosis and link actin coats to the secretory vesicle membrane to influence vesicle compression.<br />Summary: Myosin 1b and 1c localize to fused secretory vesicles and influence the compression of the actin coat during exocytosis.
- Subjects :
- Male
0301 basic medicine
Bodily Secretions
Myosin 1
Myosin light-chain kinase
macromolecular substances
Biology
Microfilament
Membrane Fusion
Exocytosis
Rats, Sprague-Dawley
Myosin Type I
03 medical and health sciences
Myosin
Animals
Cells, Cultured
Surfactant secretion
Secretory Vesicles
Actin remodeling
Pulmonary Surfactants
Cell Biology
Kiss-and-run fusion
Actin cytoskeleton
Secretory Vesicle
Rats
Cell biology
Actin Cytoskeleton
030104 developmental biology
Alveolar Epithelial Cells
Research Article
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....95bc738e77488addd42b868444b97f56