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Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin
- Source :
- Memórias do Instituto Oswaldo Cruz., Vol 114, Iss 0, Memórias do Instituto Oswaldo Cruz, Memórias do Instituto Oswaldo Cruz, Volume: 114, Article number: e180529, Published: 30 MAY 2019
- Publisher :
- Instituto Oswaldo Cruz, Ministério da Saúde
-
Abstract
- Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eggs when applied in various combinations was also examined. Nine observers registered the presence or absence of 6 different criteria in 100 eggs using a microscope at 100x magnification. Ninhydrin purple, which was frequently observed, was the criterion associated with the lowest inter-observer variability. At least three criteria were associated with a significantly better performance in egg identification. In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments.
- Subjects :
- Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
Short Communication
lcsh:QR1-502
Schistosomiasis
Biology
lcsh:Microbiology
Feces
chemistry.chemical_compound
Reference Values
schistosomiasis
parasitic diseases
medicine
Animals
ninhydrin
Parasite Egg Count
Ovum
Chromatography
Reproducibility of Results
Schistosoma mansoni
egg-detection
medicine.disease
biology.organism_classification
Staining
chemistry
coproparasitology
Ninhydrin
Indicators and Reagents
Subjects
Details
- Language :
- English
- ISSN :
- 16788060
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Memórias do Instituto Oswaldo Cruz.
- Accession number :
- edsair.doi.dedup.....95bbe55561ab500c30fa44f1333e160f