Back to Search
Start Over
Coral transcriptome and bacterial community profiles reveal distinct Yellow Band Disease states in Orbicella faveolata
- Source :
- The ISME journal, vol 8, iss 12
- Publication Year :
- 2014
- Publisher :
- Nature Publishing Group, 2014.
-
Abstract
- Coral diseases impact reefs globally. Although we continue to describe diseases, little is known about the etiology or progression of even the most common cases. To examine a spectrum of coral health and determine factors of disease progression we examined Orbicella faveolata exhibiting signs of Yellow Band Disease (YBD), a widespread condition in the Caribbean. We used a novel combined approach to assess three members of the coral holobiont: the coral-host, associated Symbiodinium algae, and bacteria. We profiled three conditions: (1) healthy-appearing colonies (HH), (2) healthy-appearing tissue on diseased colonies (HD), and (3) diseased lesion (DD). Restriction fragment length polymorphism analysis revealed health state-specific diversity in Symbiodinium clade associations. 16S ribosomal RNA gene microarrays (PhyloChips) and O. faveolata complimentary DNA microarrays revealed the bacterial community structure and host transcriptional response, respectively. A distinct bacterial community structure marked each health state. Diseased samples were associated with two to three times more bacterial diversity. HD samples had the highest bacterial richness, which included components associated with HH and DD, as well as additional unique families. The host transcriptome under YBD revealed a reduced cellular expression of defense- and metabolism-related processes, while the neighboring HD condition exhibited an intermediate expression profile. Although HD tissue appeared visibly healthy, the microbial communities and gene expression profiles were distinct. HD should be regarded as an additional (intermediate) state of disease, which is important for understanding the progression of YBD.
- Subjects :
- Technology
Montastraea faveolata
PhyloChip
Microbiology
Transcriptome
Symbiodinium
Genetics
2.2 Factors relating to the physical environment
Animals
Aetiology
Gene
Ecology, Evolution, Behavior and Systematics
Orbicella faveolata
biology
Bacteria
Prevention
Human Genome
Biological Sciences
biology.organism_classification
Anthozoa
Holobiont
Alveolata
Original Article
16S rRNA gene
DNA microarray
Restriction fragment length polymorphism
coral reefs
Yellow-band disease
yellow band blotch disease
Environmental Sciences
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- The ISME journal, vol 8, iss 12
- Accession number :
- edsair.doi.dedup.....95b67b6b9829a4b99efcd9c9c38be02e