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Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells

Authors :
Muniyandi Jeyaraj
Vasuki Eppakayala
Jae Woong Han
Jin-Hoi Kim
Sangiliyandi Gurunathan
Source :
BioMed Research International, BioMed Research International, Vol 2013 (2013)
Publication Year :
2013
Publisher :
Hindawi Limited, 2013.

Abstract

Silver nanoparticles (AgNPs) have been used as an antimicrobial and disinfectant agents. However, there is limited information about antitumor potential. Therefore, this study focused on determining cytotoxic effects of AgNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death. Herein, we developed a green method for synthesis of AgNPs using culture supernatant ofBacillus funiculus, and synthesized AgNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, particle size analyzer, and transmission electron microscopy (TEM). The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress. MDA-MB-231 breast cancer cells were treated with various concentrations of AgNPs (5 to 25 μg/mL) for 24 h. We found that AgNPs inhibited the growth in a dose-dependent manner using MTT assay. AgNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH), caspase-3, reactive oxygen species (ROS) generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. The present results showed that AgNPs might be a potential alternative agent for human breast cancer therapy.

Details

ISSN :
23146141 and 23146133
Volume :
2013
Database :
OpenAIRE
Journal :
BioMed Research International
Accession number :
edsair.doi.dedup.....95b21f62dd4bd5b1f6d6de5c394c3363
Full Text :
https://doi.org/10.1155/2013/535796