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Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells
- Source :
- BioMed Research International, BioMed Research International, Vol 2013 (2013)
- Publication Year :
- 2013
- Publisher :
- Hindawi Limited, 2013.
-
Abstract
- Silver nanoparticles (AgNPs) have been used as an antimicrobial and disinfectant agents. However, there is limited information about antitumor potential. Therefore, this study focused on determining cytotoxic effects of AgNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death. Herein, we developed a green method for synthesis of AgNPs using culture supernatant ofBacillus funiculus, and synthesized AgNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, particle size analyzer, and transmission electron microscopy (TEM). The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress. MDA-MB-231 breast cancer cells were treated with various concentrations of AgNPs (5 to 25 μg/mL) for 24 h. We found that AgNPs inhibited the growth in a dose-dependent manner using MTT assay. AgNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH), caspase-3, reactive oxygen species (ROS) generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. The present results showed that AgNPs might be a potential alternative agent for human breast cancer therapy.
- Subjects :
- Pathology
medicine.medical_specialty
Silver
Article Subject
Cell Survival
lcsh:Medicine
Metal Nanoparticles
Apoptosis
Breast Neoplasms
General Biochemistry, Genetics and Molecular Biology
Silver nanoparticle
Microscopy, Electron, Transmission
Cell Line, Tumor
medicine
Humans
MTT assay
Viability assay
Fragmentation (cell biology)
Cytotoxicity
General Immunology and Microbiology
Chemistry
lcsh:R
General Medicine
Oxidative Stress
Biochemistry
Cell culture
Cancer cell
Female
Reactive Oxygen Species
Research Article
Subjects
Details
- ISSN :
- 23146141 and 23146133
- Volume :
- 2013
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....95b21f62dd4bd5b1f6d6de5c394c3363
- Full Text :
- https://doi.org/10.1155/2013/535796