Back to Search
Start Over
Extensive intragenic recombination and patterns of linkage disequilibrium at the CSN3 locus in European rabbit
- Source :
- Genetics Selection Evolution, Vol 39, Iss 3, Pp 341-352 (2007), Genetics, Selection, Evolution : GSE, Genetics Selection Evolution, Genetics Selection Evolution, BioMed Central, 2007, 39 (3), pp.341-352
- Publication Year :
- 2007
- Publisher :
- BMC, 2007.
-
Abstract
- Kappa-casein (CSN3) plays an important role in stabilising the Ca-sensitive caseins in the micelle. The European rabbit (Oryctolagus cuniculus) CSN3 has previously been shown to possess two alleles (A and B), which differ deeply in their intronic regions (indels of 100 and 1550 nucleotides in introns 1 and 4, respectively). Furthermore, a correlation between several reproductive performance traits and the different alleles was described. However, all these data were exclusively collected in rabbit domestic breeds, preventing a deeper understanding of the extensive polymorphism observed in the CSN3 gene. Additionally, the techniques available for the typing of both indel polymorphisms were until now not suitable for large-scale studies. In this report, we describe a simple, PCR-based typing method to distinguish rabbit CSN3 alleles. We analyse both ancient wild rabbit populations from the Iberian Peninsula and France, and the more recently derived English wild rabbits and domestic stocks. A new allele (C) showing another major indel (250 bp) in intron 1 was found, but exclusively detected in Iberian wild rabbits. In addition, our survey revealed the occurrence of new haplotypes in wild populations, suggesting that intragenic recombination is important in creating genetic diversity at this locus. This easy and low cost single-step PCR-based method results in an improvement over previous described techniques, can be easily set up in a routine molecular laboratory and would probably be a valuable tool in the management of rabbit domestic breeds.
- Subjects :
- Linkage disequilibrium
Population genetics
Polymerase Chain Reaction
intragenic recombination
Gene Frequency
Genetics(clinical)
ComputingMilieux_MISCELLANEOUS
lcsh:SF1-1100
Genetics
Recombination, Genetic
0303 health sciences
Genome
Caseins
CSN3 gene
04 agricultural and veterinary sciences
General Medicine
Exons
Animals, Domestic
Rabbits
European rabbit
Nucleic Acid Amplification Techniques
Genotype
lcsh:QH426-470
Molecular Sequence Data
Locus (genetics)
[SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics
Biology
03 medical and health sciences
Species Specificity
Genetic variation
biology.domesticated_animal
Animals
Allele
Indel
Ecology, Evolution, Behavior and Systematics
Alleles
030304 developmental biology
Polymorphism, Genetic
Base Sequence
Research
Haplotype
0402 animal and dairy science
Genetic Variation
DNA
040201 dairy & animal science
Introns
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
lcsh:Genetics
Long Interspersed Nucleotide Elements
indel polymorphism
Haplotypes
Animal Science and Zoology
lcsh:Animal culture
Gene Deletion
linkage disequilibrium
Subjects
Details
- Language :
- German
- ISSN :
- 12979686 and 0999193X
- Volume :
- 39
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Genetics Selection Evolution
- Accession number :
- edsair.doi.dedup.....95b0b9812cc9024f403a1520b9736dab