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Involvement of caveolin-2 in caveolar biogenesis in MDCK cells
- Source :
- ResearcherID
- Publisher :
- Published by Elsevier B.V.
-
Abstract
- Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells caveolin-1 and -2 are found together on basolateral caveolae whereas the apical membrane, where only caveolin-1 is present, lacks caveolae. Expression of a caveolin mutant prevented the formation of the large caveolin-1/-2 hetero-oligomeric complexes, and led to intracellular retention of caveolin-2 and disappearance of caveolae from the basolateral membrane. Correspondingly, in MDCK cells over-expressing caveolin-2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin-2 in caveolar biogenesis.
- Subjects :
- Caveolin 2
Biophysics
Fluorescent Antibody Technique
Biology
Caveolae
Biochemistry
Caveolins
03 medical and health sciences
Dogs
Caveolin-2
Caveolin-1
Structural Biology
Caveolin
Genetics
Animals
Electrophoresis, Gel, Two-Dimensional
Molecular Biology
030304 developmental biology
Epithelial polarity
0303 health sciences
Vesicle
030302 biochemistry & molecular biology
Caveola
Cell Biology
Raft
Apical membrane
Cell biology
Microscopy, Electron
Caveolin 1
MDCK
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....9599d0a5f0eead69f9f56639193bc330
- Full Text :
- https://doi.org/10.1016/S0014-5793(03)00135-2